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Cattle IFNa (Interferon Alpha) ELISA Kit

CAT: 0855-ELK5981-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK5981-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle IFNa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle IFNa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle IFNa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle IFNa in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
IFNα1; IFL; LeIF D; IFN; IFN-Alpha; IFNα13; IFN-A; IFNαP22; IFN-Alpha 1b; Interferon Alpha 1b; Interferon, Leukocytic; IFN, Leukocyte; Interferon alpha-D
UniProt
P07348
Reactivity
Cattle
Field of Research
Cytokine; Infection immunity
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle IFNa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle IFNa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle IFNa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle IFNa in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
1000 pg/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
15.63-1000 pg/mL
Sensitivity
5.4 pg/mL

UniProtKB · P07348

Interferon alpha-1

IFNA1_BOVIN · Bos taurus

View on UniProt ↗
Primary accession
P07348
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
—
Protein existence
3: Inferred from homology
Organism
Bos taurus (Bovine)
Taxonomy ID
9913
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

Biological process

Antiviral defense

Molecular function

Cytokine

PTM

Disulfide bond

Technical term

Reference proteome

Cellular component

Secreted

Domain

Signal

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