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Human CSN1 (Casein Alpha) ELISA Kit

CAT: 0855-ELK4820-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK4820-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CSN1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CSN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CSN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CSN1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
CASa; CSN1; CSN1S1; Casein Alpha S1; Caseinate; Caseine/caséine/Kasein/caseína; Alpha-S1-casein; Casoxin-D
UniProt
P47710
Reactivity
Human
Field of Research
Signal transduction
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CSN1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CSN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CSN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CSN1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
100 ng/mL
Sample Type
Tissue homogenates, cell lysates, cell culture supernates, breast milk and other biological fluids
Detection Range
1.57-100 ng/mL
Sensitivity
0.55 ng/mL

UniProtKB · P47710

Alpha-S1-casein

CASA1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P47710
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
CSN1S1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
A1A510, A1A511, E9PB60, Q4PNR5
Protein keywords

Coding sequence diversity

Alternative splicing

Technical term

Direct protein sequencingProteomics identificationReference proteome

PTM

Disulfide bondPhosphoprotein

Molecular function

Milk protein

Cellular component

Secreted

Domain

Signal

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