10X PCR Buffer without MgCl2 Solution

10X PCR Buffer without MgCl2_x000D_ Catalog number: B2010121_x000D_ Lot number: Batch Dependent_x000D_ Expiration Date: Batch dependent_x000D_ Volume/Weight: 4 x 1 mL_x000D_ pH: 8.3_x000D_ Supplied as: 10X concentrate (dilute to 1X with other PCR components before use)_x000D_ Appearance: Clear solution_x000D_ Applications: optimized buffer for routine PCR (dilute to 1X and add 1-4 mM MgCl2, 200 uM each dNTP)._x000D_ Storage: −20°C_x000D_ Keywords: PCR buffer without magnesium chloride_x000D_ Grade: Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered on a 0.22 um._x000D_ _x000D_ References:_x000D_ 1: Matsuda K. PCR-Based Detection Methods for Single-Nucleotide Polymorphism or_x000D_ Mutation: Real-Time PCR and Its Substantial Contribution Toward Technological_x000D_ Refinement. Adv Clin Chem. 2017;80:45-72._x000D_ _x000D_ 2: Smith CJ, Osborn AM. Advantages and limitations of quantitative PCR_x000D_ (Q-PCR)-based approaches in microbial ecology. FEMS Microbiol Ecol. 2009_x000D_ Jan;67(1):6-20._x000D_ _x000D_ 3: Romsos EL, Vallone PM. Rapid PCR of STR markers: Applications to human_x000D_ identification. Forensic Sci Int Genet. 2015 Sep;18:90-9._x000D_ _x000D_ 4: Krawczyk B, Kur J, Stojowska-Swędrzyńska K, Śpibida M. Principles and_x000D_ applications of Ligation Mediated PCR methods for DNA-based typing of microbial_x000D_ organisms. Acta Biochim Pol. 2016;63(1):39-52._x000D_ _x000D_ 5: Green MR, Sambrook J. Analysis and Normalization of Real-Time Polymerase_x000D_ Chain Reaction (PCR) Experimental Data. Cold Spring Harb Protoc. 2018 Oct_x000D_ 1;2018(10)._x000D_ _x000D_ 6: Hapuarachchi CT, Jeffery KJM, Bowler ICJW. Stool PCR may not be a substitute_x000D_ for enrichment culture for the detection of salmonella. J Med Microbiol. 2019_x000D_ Mar;68(3):395-397._x000D_ _x000D_ 7: Ehnert S, Linnemann C, Braun B, Botsch J, Leibiger K, Hemmann P, Nussler AAK._x000D_ One-Step ARMS-PCR for the Detection of SNPs-Using the Example of the_x000D_ PADI4 Gene. Methods Protoc. 2019 Jul 25;2(3):63._x000D_ _x000D_ 8: Dahiya B, Mehta PK. Detection of potential biomarkers associated with_x000D_ outrageous diseases and environmental pollutants by nanoparticle-based immuno-_x000D_ PCR assays. Anal Biochem. 2019 Dec 15;587:113444._x000D_ _x000D_ 9: Doyle L, Vogel S, Procop GW. Pneumocystis PCR: It Is Time to Make PCR_x000D_ the Test of Choice. Open Forum Infect Dis. 2017 Sep 2;4(4):ofx193._x000D_ _x000D_ 10: Amoupour M, Nezamzadeh F, Bialvaei AZ, Sedighi M, Jazi FM, Alikhani MY,_x000D_ Mirnejad R. Differentiation of Brucella abortus and B. melitensis_x000D_ biovars using PCR-RFLP and REP-PCR. New Microbes New Infect. 2019 Aug_x000D_ 22;32:100589._x000D_ _x000D_ Products Related to 10X PCR Buffer without MgCl2 Biochemical Buffers and Solutions

Catalog Number: B2010121 (4 x 1 mL)

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