Tris Tricine SDS Buffer 10X Solution

• Description:

  Tris-Tricine-SDS Buffer 10X_x000D_ Catalog number: B2010112_x000D_ Lot number: Batch Dependent_x000D_ Expiration Date: Batch dependent_x000D_ Volume/Weight: 100 mL_x000D_ pH: 8.2_x000D_ Supplied as: 10X concentrate (dilute to 1X with di-water before use)_x000D_ Appearance: Clear solution_x000D_ Applications: running buffer for polyacrylamide based separation of bronchoalveolar lavage fluids as well as low molecular weight proteins._x000D_ Storage: Room temperature_x000D_ Keywords: Tris-Tricine-SDS, TTS, Low molecular weight SDS-PAGE_x000D_ Grade: Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered on a 0.22 um._x000D_ _x000D_ References:_x000D_ 1: Fernandez-Patron C, Hardy E, Sosa A, Seoane J, Castellanos L. Double staining_x000D_ of coomassie blue-stained polyacrylamide gels by imidazole-sodium dodecyl_x000D_ sulfate-zinc reverse staining: sensitive detection of coomassie blue-undetected_x000D_ proteins. Anal Biochem. 1995 Jan 1;224(1):263-9._x000D_ _x000D_ 2: Brum AM, Thomas AD, Sabeur K, Ball BA. Evaluation of Coomassie blue staining_x000D_ of the acrosome of equine and canine spermatozoa. Am J Vet Res. 2006_x000D_ Feb;67(2):358-62._x000D_ _x000D_ 3: Marshall T, Williams KM. Total protein determination in urine: elimination of_x000D_ a differential response between the coomassie blue and pyrogallol red protein_x000D_ dye-binding assays. Clin Chem. 2000 Mar;46(3):392-8._x000D_ _x000D_ 4: Lim CW, Chisnall WN, Stokes YM, Pratt R, Crooke MJ. Effects of sodium_x000D_ dodecylsulphate, dye concentration and paraprotein on coomassie blue dye-binding_x000D_ assays for protein in urine. Clin Biochem. 1988 Oct;21(5):277-81._x000D_ _x000D_ 5: Fujita T, Toda T, Ohashi M. Enzyme-linked immunodetection of proteins on_x000D_ Coomassie blue-stained two-dimensional cellulose acetate membranes. Anal_x000D_ Biochem. 1986 Nov 15;159(1):8-11._x000D_ _x000D_ 6: Fountoulakis M, Juranville JF, Manneberg M. Comparison of the Coomassie_x000D_ brilliant blue, bicinchoninic acid and Lowry quantitation assays, using non-_x000D_ glycosylated and glycosylated proteins. J Biochem Biophys Methods. 1992_x000D_ Jun;24(3-4):265-74._x000D_ _x000D_ 7: Spector T. Refinement of the coomassie blue method of protein quantitation. A_x000D_ simple and linear spectrophotometric assay for less than or equal to 0.5 to 50_x000D_ microgram of protein. Anal Biochem. 1978 May;86(1):142-6._x000D_ _x000D_ Products Related to Tris-Tricine-SDS Buffer 10X: Buffers and Solutions

• Short Description:

  Catalog Number: B2010112 (100 mL)

• Weight:

  5.5

• Length:

  5

• Width:

  3

• Height:

  3

• Height :

  3