Tris Tricine SDS Buffer 10X Solution

• Description:

  Tris-Tricine-SDS Buffer 10X_x000D_ Catalog number: B2010112_x000D_ Lot number: Batch Dependent_x000D_ Expiration Date: Batch dependent_x000D_ Volume/Weight: 100 mL_x000D_ pH: 8.2_x000D_ Supplied as: 10X concentrate (dilute to 1X with di-water before use)_x000D_ Appearance: Clear solution_x000D_ Applications: running buffer for polyacrylamide based separation of bronchoalveolar lavage fluids as well as low molecular weight proteins._x000D_ Storage: Room temperature_x000D_ Keywords: Tris-Tricine-SDS, TTS, Low molecular weight SDS-PAGE_x000D_ Grade: Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered on a 0.22 um._x000D_ _x000D_ References:_x000D_ 1: Fernandez-Patron C, Hardy E, Sosa A, Seoane J, Castellanos L. Double staining_x000D_ of coomassie blue-stained polyacrylamide gels by imidazole-sodium dodecyl_x000D_ sulfate-zinc reverse staining: sensitive detection of coomassie blue-undetected_x000D_ proteins. Anal Biochem. 1995 Jan 1;224(1):263-9._x000D_ _x000D_ 2: Brum AM, Thomas AD, Sabeur K, Ball BA. Evaluation of Coomassie blue staining_x000D_ of the acrosome of equine and canine spermatozoa. Am J Vet Res. 2006_x000D_ Feb;67(2):358-62._x000D_ _x000D_ 3: Marshall T, Williams KM. Total protein determination in urine: elimination of_x000D_ a differential response between the coomassie blue and pyrogallol red protein_x000D_ dye-binding assays. Clin Chem. 2000 Mar;46(3):392-8._x000D_ _x000D_ 4: Lim CW, Chisnall WN, Stokes YM, Pratt R, Crooke MJ. Effects of sodium_x000D_ dodecylsulphate, dye concentration and paraprotein on coomassie blue dye-binding_x000D_ assays for protein in urine. Clin Biochem. 1988 Oct;21(5):277-81._x000D_ _x000D_ 5: Fujita T, Toda T, Ohashi M. Enzyme-linked immunodetection of proteins on_x000D_ Coomassie blue-stained two-dimensional cellulose acetate membranes. Anal_x000D_ Biochem. 1986 Nov 15;159(1):8-11._x000D_ _x000D_ 6: Fountoulakis M, Juranville JF, Manneberg M. Comparison of the Coomassie_x000D_ brilliant blue, bicinchoninic acid and Lowry quantitation assays, using non-_x000D_ glycosylated and glycosylated proteins. J Biochem Biophys Methods. 1992_x000D_ Jun;24(3-4):265-74._x000D_ _x000D_ 7: Spector T. Refinement of the coomassie blue method of protein quantitation. A_x000D_ simple and linear spectrophotometric assay for less than or equal to 0.5 to 50_x000D_ microgram of protein. Anal Biochem. 1978 May;86(1):142-6._x000D_ _x000D_ Products Related to Tris-Tricine-SDS Buffer 10X: Buffers and Solutions

• Short Description:

  Catalog Number: B2010112 (100 mL)_x000D_ Tris-Tricine-SDS Buffer 10X is a high quality research product used as running buffer for polyacrylamide based separation of bronchoalveolar lavage fluids as well as low molecular weight proteins. Custom bulk orders of this product are available upon request.

• Weight:

  5.5

• Length:

  5

• Width:

  3

• Height :

  3