CIAP Reaction Buffer (10X Solution)

• Short Description:

  Catalog Number: B2010100 (2 mL)_x000D_ _x000D_ Calf Intestinal Alkaline Phosphatase Reaction Buffer is a highly optimized, high quality, reproducible reaction buffer for Calf Intestinal Alkaline Phosphatase (CIAP). Supplied as 10X, it minimizes sample dilution during enzymatic reactions.

• Description:

  CIAP Reaction Buffer (10X)_x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_

  Catalog number:	B2010100
  Lot number:	Batch Dependent
  Expiration date:	Batch Dependent
  Volume/Weight	2 mL
  pH	9.3
  Supplied as	10X
  Appearance	Clear Solution
  Applications	Reaction buffer for Calf Intestinal Alkaline Phosphatase.
  Shelf-Life	1 year from date of manufacture
  Storage:	Keep at 2-8°C
  Keywords:	CIAP buffer, CIAP reaction solution, CIAP reaction buffer
  Grade	Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered on a 0.22 um.
  References	1: Channar PA, Shah SJ, Hassan S, Nisa ZU, Lecka J, Sévigny J, Bajorath J, Saeed_x000D_ A, Iqbal J. Isonicotinohydrazones as inhibitors of alkaline phosphatase and_x000D_ ecto-5'-nucleotidase. Chem Biol Drug Des. 2017 Mar;89(3):365-370._x000D_ _x000D_ 2: Khattab M, Pfleiderer G. Alkaline phosphatase of human and calf small_x000D_ intestine. Purification and immunochemical characterization. Hoppe Seylers Z_x000D_ Physiol Chem. 1976 Mar;357(3):377-91._x000D_ _x000D_ 3: Ejaz SA, Saeed A, Siddique MN, Nisa ZU, Khan S, Lecka J, Sévigny J, Iqbal J._x000D_ Synthesis, characterization and biological evaluation of novel chalcone_x000D_ sulfonamide hybrids as potent intestinal alkaline phosphatase inhibitors. Bioorg_x000D_ Chem. 2017 Feb;70:229-236._x000D_ _x000D_ 4: Ensinger HA, Pauly HE, Pfleiderer G, Stiefel T. The role of Zn(II) in calf_x000D_ intestinal alkaline phosphatase studied by the influence of chelating agents and_x000D_ chemical modification of histidine residues. Biochim Biophys Acta. 1978 Dec_x000D_ 8;527(2):432-41._x000D_ _x000D_ 5: Alam SN, Yammine H, Moaven O, Ahmed R, Moss AK, Biswas B, Muhammad N, Biswas_x000D_ R, Raychowdhury A, Kaliannan K, Ghosh S, Ray M, Hamarneh SR, Barua S, Malo NS,_x000D_ Bhan AK, Malo MS, Hodin RA. Intestinal alkaline phosphatase prevents antibiotic-_x000D_ induced susceptibility to enteric pathogens. Ann Surg. 2014 Apr;259(4):715-22._x000D_ _x000D_ _x000D_ 6: Igunnu A, Osalaye DS, Olorunsogo OO, Malomo SO, Olorunniji FJ. Distinct metal_x000D_ ion requirements for the phosphomonoesterase and phosphodiesterase activities of_x000D_ calf intestinal alkaline phosphatase. Open Biochem J. 2011;5:67-72._x000D_ _x000D_ 7: Fernley HN, Bisaz S. Studies on alkaline phosphatase. Phosphorylation of_x000D_ calf-intestinal alkaline phosphatase by 32P-labelled pyrophosphate. Biochem J._x000D_ 1968 Mar;107(2):279-83._x000D_ _x000D_ 8: Chaudhuri G, Chatterjee S, Venu-Babu P, Ramasamy K, Thilagaraj WR. Kinetic_x000D_ behaviour of calf intestinal alkaline phosphatase with pNPP. Indian J Biochem_x000D_ Biophys. 2013 Feb;50(1):64-71. PMID: 23617076._x000D_ _x000D_ 9: Yan S, Liu Y, Tian X, Zhang Y, Zhou H. Effect of extraneous zinc on calf_x000D_ intestinal alkaline phosphatase. J Protein Chem. 2003 May;22(4):371-5._x000D_ _x000D_ 10: Zhang YX, Song XH, Yan SL, Zhou HM. The unfolding intermediate state of calf_x000D_ intestinal alkaline phosphatase during denaturation in guanidine solutions. J_x000D_ Protein Chem. 2003 Jul;22(5):405-9.
  Related Products	Biochemical Buffers and Solutions

• Weight:

  0.15

• Length:

  2

• Width:

  0.5

• Height :

  0.5