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Reverse Transcription Buffer (10X)

CAT: 0952-B2010084Size: 2 mLDry Ice: NoHazardous: No
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CAT#:0952-B2010084Size:2 mL
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Description
Reverse Transcription Buffer (10X)_x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_
Catalog number:B2010084
Lot number:Batch Dependent
Expiration date:Batch Dependent
Volume/Weight2 mL
pH9
Supplied as10 X concentrated solution. Dilute with di-water to 1X working solution.
AppearanceClear solution
ApplicationsUseful for reverse transcription reactions and production of cDNA, works with AMV Reverse Transcriptase and Taq DNA polymerase. Buffer is compatible with down stream applications such as PCR.
Shelf-Life1 year from date of manufacture
Storage:Keep at 2-8°C
Keywords:Reverse transcription buffer, RT Buffer, 10X RT buffer, cDNA synthesis buffer, 10X RT buffer
GradeBiotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered on a 0.22 um.
References1: Tekeste SS, Wilkinson TA, Weiner EM, Xu X, Miller JT, Le Grice SF, Clubb RT,_x000D_ Chow SA. Interaction between Reverse Transcriptase and Integrase Is Required for_x000D_ Reverse Transcription during HIV-1 Replication. J Virol. 2015_x000D_ Dec;89(23):12058-69.2: Fungtammasan A, Tomaszkiewicz M, Campos-S√°nchez R, Eckert KA, DeGiorgio M,_x000D_ Makova KD. Reverse Transcription Errors and RNA-DNA Differences at Short Tandem_x000D_ Repeats. Mol Biol Evol. 2016 Oct;33(10):2744-58._x000D_ _x000D_ 3: Masaki Y, Ito H, Oda Y, Yamazaki K, Tago N, Ohno K, Ishii N, Tsunoda H,_x000D_ Kanamori T, Ohkubo A, Sekine M, Seio K. Enzymatic synthesis and reverse_x000D_ transcription of RNAs incorporating 2'-O-carbamoyl uridine triphosphate. Chem_x000D_ Commun (Camb). 2016 Oct 25;52(87):12889-12892._x000D_ 4: Cosnefroy O, Murray PJ, Bishop KN. HIV-1 capsid uncoating initiates after the_x000D_ first strand transfer of reverse transcription. Retrovirology. 2016 Aug_x000D_ 22;13(1):58._x000D_ 5: Zhao LM, Li G, Gao Y, Zhu YR, Liu J, Zhu XP. Reverse transcription loop-_x000D_ mediated isothermal amplification assay for detecting tomato chlorosis virus. J_x000D_ Virol Methods. 2015 Mar;213:93-7._x000D_ 6: Chen L, Jiao Z, Liu D, Liu X, Xia Z, Deng C, Zhou T, Fan Z. One-step reverse_x000D_ transcription loop-mediated isothermal amplification for the detection of Maize_x000D_ chlorotic mottle virus in maize. J Virol Methods. 2017 Feb;240:49-53._x000D_ _x000D_ 7: Wang FX, Yuan DY, Jin YN, Hu L, Sun ZY, He Q, Zhao SH, Zhan SB, Wen YJ._x000D_ Reverse Transcription Cross-Priming Amplification-Nucleic Acid Test Strip for_x000D_ Rapid Detection of Porcine Epidemic Diarrhea Virus. Sci Rep. 2016 Apr_x000D_ 19;6:24702.
Related ProductsBiochemical Buffers and Solutions
Short Description
Catalog Number: B2010084 (2 mL)
Weight
0.15
Length
2
Width
0.5
Height
0.5

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