Phospho-LRRK2 (S935) Recombinant Rabbit Monoclonal Antibody [JE42-11]

Phospho-LRRK2 (S935) Recombinant Rabbit Monoclonal Antibody [JE42-11]

• Description: The LRRK2 gene encodes a protein with an armadillo repeats (ARM) region, an ankyrin repeat (ANK) region, a leucine-rich repeat (LRR) domain, a kinase domain, a RAS domain, a GTPase domain, and a WD40 domain. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane. LRRK2 interacts with the C-terminal R2 RING finger domain of parkin, and parkin interacted with the COR domain of LRRK2. Expression of mutant LRRK2 induced apoptotic cell death in neuroblastoma cells and in mouse cortical neurons. Expression of LRRK2 mutants implicated in autosomal dominant Parkinson's disease causes shortening and simplification of the dendritic tree in vivo and in cultured neurons. This is mediated in part by alterations in macroautophagy, and can be prevented by protein kinase A regulation of the autophagy protein LC3. The G2019S and R1441C mutations elicit post-synaptic calcium imbalance, leading to excess mitochondrial clearance from dendrites by mitophagy. LRRK2 is also a substrate for chaperone-mediated autophagy.
• Product Name Alternative: augmented in rheumatoid arthritis 17 antibody AURA17 antibody Dardarin antibody Leucine rich repeat kinase 2 antibody leucine rich repeat serine threonine protein kinase 2 antibody Leucine-rich repeat serine/threonine-protein kinase 2 antibody LRRK 2 antibody LRRK2 antibody LRRK2_HUMAN antibody PARK 8 antibody PARK8 antibody RIPK7 antibody ROCO 2 antibody ROCO2 antibody
• Abbreviation: LRRK2, PARK8
• CAS Number: 9000-83-3
• Swiss Prot: Q5S007 Human, Q5S006 Mouse
• Cellular Locus: Cytoplasmic vesicle, Perikaryon, Golgi apparatus membrane, Cell projection, axon, dendrite, Endoplasmic reticulum membrane, Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane, Endosome, Lysosome, Mitochondrion outer membrane, Cytoplasm, cytoskeleton.
• Host: Rabbit
• Species Reactivity: Mouse, Human
• Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Ser935 of human LRRK2 protein.
• Isotype: IgG
• Clone: JE42-11
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IF-Cell, FC
• Positive Control: MEF treated with 1μM okadaic acid for 1 hour cell lysate, MEF treated with 100nM Calyculin A for 30 minutes cell lysate, NIH/3T3 cell lysate, MEF cells treated with 100nM Calyculin A for 30 minutes.
• Concentration: 1 mg/mL
• Dilution: WB: 1:1,000 ;IF-Cell: 1:1,000 ;FC: 1:1,000
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 286 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
• Recombinant Antibody: Yes