PSMC6 Recombinant Rabbit Monoclonal Antibody [PSH0-12]

PSMC6 Recombinant Rabbit Monoclonal Antibody [PSH0-12]

• Description: As the degradation machinery that is responsible for ~70% of intracellular proteolysis, proteasome complex (26S proteasome) plays a critical roles in maintaining the homeostasis of cellular proteome. Accordingly, misfolded proteins and damaged protein need to be continuously removed to recycle amino acids for new synthesis; in parallel, some key regulatory proteins fulfill their biological functions via selective degradation; furthermore, proteins are digested into peptides for MHC class I antigen presentation. To meet such complicated demands in biological process via spatial and temporal proteolysis, protein substrates have to be recognized, recruited, and eventually hydrolyzed in a well controlled fashion. Thus, 19S regulatory particle pertains a series of important capabilities to address these functional challenges. To recognize protein as designated substrate, 19S complex has subunits that are capable to recognize proteins with a special degradative tag, the ubiquitinylation. It also have subunits that can bind with nucleotides (e.g., ATPs) in order to facilitate the association between 19S and 20S particles, as well as to cause confirmation changes of alpha subunit C-terminals that form the substate entrance of 20S complex. The ATPases subunits assemble into a six-membered ring with a sequence of Rpt1–Rpt5–Rpt4–Rpt3–Rpt6–Rpt2, which interacts with the seven-membered alpha ring of 20S core particle and establishes an asymmetric interface between the 19S RP and the 20S CP. Three C-terminal tails with HbYX motifs of distinct Rpt ATPases insert into pockets between two defined alpha subunits of the CP and regulate the gate opening of the central channels in the CP alpha ring. Evidence showed that ATPase subunit Rpt5, along with other ubuiqintinated 19S proteasome subunits (Rpn13, Rpn10) and the deubiquitinating enzyme Uch37, can be ubiquitinated in situ by proteasome-associating ubiquitination enzymes. Ubiquitination of proteasome subunits can regulates proteasomal activity in response to the alteration of cellular ubiquitination levels.
• Product Name Alternative: 26S protease regulatory subunit 10B antibody 26S protease regulatory subunit S10B antibody 26S proteasome AAA-ATPase subunit RPT4 antibody CADP44 antibody P44 antibody Proteasome 26S subunit ATPase 6 antibody Proteasome subunit p42 antibody PRS10_HUMAN antibody PSMC6 antibody Rpt4 antibody SUG2 antibody
• Abbreviation: PSMC6, SUG2
• CAS Number: 9000-83-3
• Swiss Prot: P62333 Human
• Cellular Locus: Cytoplasm, Nucleus.
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant protein within human PSMC6 aa 1-200 / 389.
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IHC-P, FC
• Positive Control: Hela cell lysate, 293T cell lysate, SH-SY5Y cell lysate, HepG2 cell lysate, PC-12 cell lysate, NIH/3T3 cell lysate, mouse kidney tissue lysate, rat kidney tissue lysate, rat brain tissue lysate, human testis tissue, SH-SY5Y, NIH/3T3, PC-12.
• Concentration: 1 mg/mL
• Dilution: WB: 1:1,000 ;IHC-P: 1:400 ;FC: 1:500-1:1,000
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 44 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
• Recombinant Antibody: Yes