eIF4E Recombinant Rabbit Monoclonal Antibody [JE60-70]

eIF4E Recombinant Rabbit Monoclonal Antibody [JE60-70]

• Description: Eukaryotic translation initiation factor 4E, also known as eIF4E, is a protein that in humans is encoded by the EIF4E gene. Most eukaryotic cellular mRNAs are blocked at their 5'-ends with the 7-methyl-guanosine five-prime cap structure, m7GpppX (where X is any nucleotide). This structure is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export. eIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of the eIF4F pre-initiation complex. Almost all cellular mRNA require eIF4E in order to be translated into protein. The eIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of eIF4F are a 47-kD polypeptide, termed eIF4A,that possesses ATPase and RNA helicase activities, and a 220-kD scaffolding polypeptide, eIF4G. Some viruses cut eIF4G in such a way that the eIF4E binding site is removed and the virus is able to translate its proteins without eIF4E. Also some cellular proteins, the most notable being heat shock proteins, do not require eIF4E in order to be translated. Both viruses and cellular proteins achieve this through an internal ribosome entry site in the RNA.
• Product Name Alternative: AUTS19 antibody CBP antibody eIF 4E antibody eIF 4F 25 kDa subunit antibody EIF 4F antibody eIF-4E antibody eIF-4F 25 kDa subunit antibody eIF4E antibody EIF4E1 antibody EIF4EL1 antibody EIF4F antibody Eukaryotic translation initiation factor 4 E antibody Eukaryotic translation initiation factor 4E antibody Eukaryotic translation initiation factor 4E like 1 antibody IF4E_HUMAN antibody Messanger RNA Cap Binding Protein eIF 4E antibody MGC111573 antibody mRNA cap binding protein antibody mRNA cap-binding protein antibody
• Abbreviation: EIF4E, EIF4EL1, EIF4F
• CAS Number: 9000-83-3
• Swiss Prot: P06730 Human, P63073 Mouse
• Cellular Locus: Cytoplasm, P-body, Stress granule, Nucleus.
• Host: Rabbit
• Species Reactivity: Human, Mouse
• Immunogen: Recombinant protein within Human eIF4E aa 118-217 / 217.
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IHC-P, IF-Cell, FC
• Positive Control: HL-60 cell lysate, Daudi cell lysate, human testis tissue, human colon carcinoma tissue, human breast carcinoma tissue, mouse hippocampus tissue, mouse brain tissue, SK-Br-3.
• Concentration: 1 mg/mL
• Dilution: WB: 1:500;IHC-P: 1:200;IF-Cell: 1:50;FC: 1:500-1:1,000
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 25 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
• Recombinant Antibody: Yes