YTHDF2 Rabbit Polyclonal Antibody

YTHDF2 Rabbit Polyclonal Antibody

• Description: Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, and regulates their stability. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in mRNA stability and processing. Acts as a regulator of mRNA stability by promoting degradation of m6A-containing mRNAs via interaction with the CCR4-NOT and ribonuclease P/MRP complexes, depending on the context. The YTHDF paralogs (YTHDF1, YTHDF2 and YTHDF3) share m6A-containing mRNAs targets and act redundantly to mediate mRNA degradation and cellular differentiation. M6A-containing mRNAs containing a binding site for RIDA/HRSP12 (5'-GGUUC-3') are preferentially degraded by endoribonucleolytic cleavage: cooperative binding of RIDA/HRSP12 and YTHDF2 to transcripts leads to recruitment of the ribonuclease P/MRP complex. Other m6A-containing mRNAs undergo deadenylation via direct interaction between YTHDF2 and CNOT1, leading to recruitment of the CCR4-NOT and subsequent deadenylation of m6A-containing mRNAs. Required maternally to regulate oocyte maturation: probably acts by binding to m6A-containing mRNAs, thereby regulating maternal transcript dosage during oocyte maturation, which is essential for the competence of oocytes to sustain early zygotic development. Also required during spermatogenesis: regulates spermagonial adhesion by promoting degradation of m6A-containing transcripts coding for matrix metallopeptidases. Also involved in hematopoietic stem cells specification by binding to m6A-containing mRNAs, leading to promote their degradation. Also acts as a regulator of neural development by promoting m6A-dependent degradation of neural development-related mRNA targets.
• Product Name Alternative: 9430020E02Rik antibody CAHL antibody CLL associated antigen KW 14 antibody CLL-associated antigen KW-14 antibody HGRG8 antibody High glucose regulated protein 8 antibody High-glucose-regulated protein 8 antibody NY REN 2 antibody Renal carcinoma antigen NY REN 2 antibody Renal carcinoma antigen NY-REN-2 antibody YTH domain family 2 antibody YTH domain family member 2 antibody YTH domain family protein 2 antibody YTH N6 methyladenosine RNA binding protein 2 antibody YTHD2_HUMAN antibody YTHDF 2 antibody YTHDF2 antibody
• Abbreviation: DF2 HGRG8 YTHDF2
• CAS Number: 9007-83-4
• Swiss Prot: Q9Y5A9 Human, Q91YT7 Mouse
• Cellular Locus: Nucleus, cytosol.
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant protein within Full length of Human YTHDF2.
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Rabbit polyclonal Antibody
• Applications: WB, IF-Cell
• Positive Control: 293T cell lysate, PC-12 cell lysate, NIH/3T3 cell lysates, NIH/3T3.
• Concentration: 1 mg/mL
• Dilution: WB: 1:500-1:1,000 ;IF-Cell: 1:100
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 62 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
• Recombinant Antibody: No