YTHDF2 Rabbit Polyclonal Antibody

• Description:

  Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, and regulates their stability. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in mRNA stability and processing. Acts as a regulator of mRNA stability by promoting degradation of m6A-containing mRNAs via interaction with the CCR4-NOT and ribonuclease P/MRP complexes, depending on the context. The YTHDF paralogs (YTHDF1, YTHDF2 and YTHDF3) share m6A-containing mRNAs targets and act redundantly to mediate mRNA degradation and cellular differentiation. M6A-containing mRNAs containing a binding site for RIDA/HRSP12 (5'-GGUUC-3') are preferentially degraded by endoribonucleolytic cleavage: cooperative binding of RIDA/HRSP12 and YTHDF2 to transcripts leads to recruitment of the ribonuclease P/MRP complex. Other m6A-containing mRNAs undergo deadenylation via direct interaction between YTHDF2 and CNOT1, leading to recruitment of the CCR4-NOT and subsequent deadenylation of m6A-containing mRNAs. Required maternally to regulate oocyte maturation: probably acts by binding to m6A-containing mRNAs, thereby regulating maternal transcript dosage during oocyte maturation, which is essential for the competence of oocytes to sustain early zygotic development. Also required during spermatogenesis: regulates spermagonial adhesion by promoting degradation of m6A-containing transcripts coding for matrix metallopeptidases. Also involved in hematopoietic stem cells specification by binding to m6A-containing mRNAs, leading to promote their degradation. Also acts as a regulator of neural development by promoting m6A-dependent degradation of neural development-related mRNA targets.

• Product Name Alternative:

  9430020E02Rik antibody CAHL antibody CLL associated antigen KW 14 antibody CLL-associated antigen KW-14 antibody HGRG8 antibody High glucose regulated protein 8 antibody High-glucose-regulated protein 8 antibody NY REN 2 antibody Renal carcinoma antigen NY REN 2 antibody Renal carcinoma antigen NY-REN-2 antibody YTH domain family 2 antibody YTH domain family member 2 antibody YTH domain family protein 2 antibody YTH N6 methyladenosine RNA binding protein 2 antibody YTHD2_HUMAN antibody YTHDF 2 antibody YTHDF2 antibody

• Abbreviation:

  DF2 HGRG8 YTHDF2

• Swiss Prot:

  Q9Y5A9 Human, Q91YT7 Mouse

• Cellular Locus:

  Nucleus, cytosol.

• Host:

  Rabbit

• Species Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant protein within Full length of Human YTHDF2.

• Isotype:

  IgG

• Conjugation:

  Non-conjugated

• Type:

  Rabbit polyclonal Antibody

• Applications:

  WB, IF-Cell

• Positive Control:

  293T cell lysate, PC-12 cell lysate, NIH/3T3 cell lysates, NIH/3T3.

• Concentration:

  1 mg/mL

• Dilution:

  WB: 1:500-1:1,000 ;IF-Cell: 1:100

• Purity:

  Protein A affinity purified.

• Form:

  Liquid

• Buffer:

  PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• Molecular Weight:

  Predicted band size: 62 kDa

• Storage Conditions:

  Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

• Recombinant Antibody:

  No

• CAS Number:

  9007-83-4