YTHDF2 Recombinant Rabbit Monoclonal Antibody [PSH06-17]

YTHDF2 Recombinant Rabbit Monoclonal Antibody [PSH06-17]

• Description: Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, and regulates their stability. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in mRNA stability and processing. Acts as a regulator of mRNA stability by promoting degradation of m6A-containing mRNAs via interaction with the CCR4-NOT and ribonuclease P/MRP complexes, depending on the context. The YTHDF paralogs (YTHDF1, YTHDF2 and YTHDF3) share m6A-containing mRNAs targets and act redundantly to mediate mRNA degradation and cellular differentiation. M6A-containing mRNAs containing a binding site for RIDA/HRSP12 (5'-GGUUC-3') are preferentially degraded by endoribonucleolytic cleavage: cooperative binding of RIDA/HRSP12 and YTHDF2 to transcripts leads to recruitment of the ribonuclease P/MRP complex. Other m6A-containing mRNAs undergo deadenylation via direct interaction between YTHDF2 and CNOT1, leading to recruitment of the CCR4-NOT and subsequent deadenylation of m6A-containing mRNAs. Required maternally to regulate oocyte maturation: probably acts by binding to m6A-containing mRNAs, thereby regulating maternal transcript dosage during oocyte maturation, which is essential for the competence of oocytes to sustain early zygotic development. Also required during spermatogenesis: regulates spermagonial adhesion by promoting degradation of m6A-containing transcripts coding for matrix metallopeptidases. Also involved in hematopoietic stem cells specification by binding to m6A-containing mRNAs, leading to promote their degradation. Also acts as a regulator of neural development by promoting m6A-dependent degradation of neural development-related mRNA targets.
• Product Name Alternative: 9430020E02Rik antibody CAHL antibody CLL associated antigen KW 14 antibody CLL-associated antigen KW-14 antibody HGRG8 antibody High glucose regulated protein 8 antibody High-glucose-regulated protein 8 antibody NY REN 2 antibody Renal carcinoma antigen NY REN 2 antibody Renal carcinoma antigen NY-REN-2 antibody YTH domain family 2 antibody YTH domain family member 2 antibody YTH domain family protein 2 antibody YTH N6 methyladenosine RNA binding protein 2 antibody YTHD2_HUMAN antibody YTHDF 2 antibody YTHDF2 antibody
• Abbreviation: YTH domain-containing family protein 2 DF2 CLL-associated antigen KW-14 High-glucose-regulated protein 8 Renal carcinoma antigen NY-REN-2 YTHDF2 HGRG8
• CAS Number: 9000-83-3
• Swiss Prot: Q9Y5A9 Human, Q91YT7 Mouse,
• Cellular Locus: Cytoplasm. Nucleus.
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat, Monkey
• Immunogen: Synthetic peptide within Human YTHDF2 aa 519-579.
• Isotype: IgG
• Clone: PSH06-17
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IHC-P, FC
• Positive Control: HEK-293 cell lysate, HeLa cell lysate, Jurkat cell lysate, HCT 116 cell lysate, MDA-MB-231 cell lysate, COS-1 cell lysate, human testis tissue, mouse brain tissue, mouse testis tissue, rat brain tissue, rat testis tissue, HCT 116.
• Concentration: 1 mg/mL
• Dilution: WB: 1:2,000 ;IHC-P: 1:500 ;FC: 1:1,000
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 62 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
• Recombinant Antibody: Yes