YTHDF1 Rabbit Polyclonal Antibody

• Description:

  Specifically recognizes and binds N6-methyladenosine (m6A)-containing mRNAs, and regulates their stability. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in mRNA stability and processing. Acts as a regulator of mRNA stability by promoting degradation of m6A-containing mRNAs via interaction with the CCR4-NOT complex. The YTHDF paralogs (YTHDF1, YTHDF2 and YTHDF3) shares m6A-containing mRNAs targets and act redundantly to mediate mRNA degradation and cellular differentiation. Required to facilitate learning and memory formation in the hippocampus by binding to m6A-containing neuronal mRNAs. Acts as a regulator of axon guidance by binding to m6A-containing ROBO3 transcripts. Acts as a negative regulator of antigen cross-presentation in myeloid dendritic cells. In the context of tumorigenesis, negative regulation of antigen cross-presentation limits the anti-tumor response by reducing efficiency of tumor-antigen cross-presentation. Promotes formation of phase-separated membraneless compartments, such as P-bodies or stress granules, by undergoing liquid-liquid phase separation upon binding to mRNAs containing multiple m6A-modified residues: polymethylated mRNAs act as a multivalent scaffold for the binding of YTHDF proteins, juxtaposing their disordered regions and thereby leading to phase separation. The resulting mRNA-YTHDF complexes then partition into different endogenous phase-separated membraneless compartments, such as P-bodies, stress granules or neuronal RNA granules.

• Product Name Alternative:

  C20orf21 antibody DACA 1 antibody DACA-1 antibody Dermatomyositis associated with cancer putative autoantigen 1 antibody YTH domain family 1 antibody YTH domain family member 1 antibody YTH domain family protein 1 antibody YTHD1 antibody YTHD1_HUMAN antibody Ythdf1 antibody

• Abbreviation:

  DF1 YTHDF1 DACA-1 C20orf21

• Swiss Prot:

  Q9BYJ9 Human, P59326 Mouse,

• Cellular Locus:

  Cytoplasm, P-body.

• Host:

  Rabbit

• Species Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant protein within Full length of Human YTHDF1.

• Isotype:

  IgG

• Conjugation:

  Non-conjugated

• Type:

  Rabbit polyclonal Antibody

• Applications:

  WB, IF-Cell, IHC-P

• Positive Control:

  MCF-7 cell lysate, PC-12 cell lysate, NIH/3T3 cell lysates, NIH/3T3, rat brain tissue, mouse brain tissue.

• Concentration:

  1 mg/mL

• Dilution:

  WB: 1:500-1:1,000;IF-Cell: 1:100;IHC-P: 1:2,000

• Purity:

  Protein A affinity purified.

• Form:

  Liquid

• Buffer:

  PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• Molecular Weight:

  Predicted band size: 61 kDa

• Storage Conditions:

  Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

• Recombinant Antibody:

  No

• CAS Number:

  9007-83-4