YTHDF1 Rabbit Polyclonal Antibody

YTHDF1 Rabbit Polyclonal Antibody

• Description: Specifically recognizes and binds N6-methyladenosine (m6A)-containing mRNAs, and regulates their stability. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in mRNA stability and processing. Acts as a regulator of mRNA stability by promoting degradation of m6A-containing mRNAs via interaction with the CCR4-NOT complex. The YTHDF paralogs (YTHDF1, YTHDF2 and YTHDF3) shares m6A-containing mRNAs targets and act redundantly to mediate mRNA degradation and cellular differentiation. Required to facilitate learning and memory formation in the hippocampus by binding to m6A-containing neuronal mRNAs. Acts as a regulator of axon guidance by binding to m6A-containing ROBO3 transcripts. Acts as a negative regulator of antigen cross-presentation in myeloid dendritic cells. In the context of tumorigenesis, negative regulation of antigen cross-presentation limits the anti-tumor response by reducing efficiency of tumor-antigen cross-presentation. Promotes formation of phase-separated membraneless compartments, such as P-bodies or stress granules, by undergoing liquid-liquid phase separation upon binding to mRNAs containing multiple m6A-modified residues: polymethylated mRNAs act as a multivalent scaffold for the binding of YTHDF proteins, juxtaposing their disordered regions and thereby leading to phase separation. The resulting mRNA-YTHDF complexes then partition into different endogenous phase-separated membraneless compartments, such as P-bodies, stress granules or neuronal RNA granules.
• Product Name Alternative: C20orf21 antibody DACA 1 antibody DACA-1 antibody Dermatomyositis associated with cancer putative autoantigen 1 antibody YTH domain family 1 antibody YTH domain family member 1 antibody YTH domain family protein 1 antibody YTHD1 antibody YTHD1_HUMAN antibody Ythdf1 antibody
• Abbreviation: DF1 YTHDF1 DACA-1 C20orf21
• CAS Number: 9007-83-4
• Swiss Prot: Q9BYJ9 Human, P59326 Mouse,
• Cellular Locus: Cytoplasm, P-body.
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant protein within Full length of Human YTHDF1.
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Rabbit polyclonal Antibody
• Applications: WB, IF-Cell, IHC-P
• Positive Control: MCF-7 cell lysate, PC-12 cell lysate, NIH/3T3 cell lysates, NIH/3T3, rat brain tissue, mouse brain tissue.
• Concentration: 1 mg/mL
• Dilution: WB: 1:500-1:1,000;IF-Cell: 1:100;IHC-P: 1:2,000
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 61 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
• Recombinant Antibody: No