YTHDF1 Recombinant Rabbit Monoclonal Antibody [PSH0-23]

YTHDF1 Recombinant Rabbit Monoclonal Antibody [PSH0-23]

• Description: Specifically recognizes and binds N6-methyladenosine (m6A)-containing mRNAs, and regulates their stability. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in mRNA stability and processing. Acts as a regulator of mRNA stability by promoting degradation of m6A-containing mRNAs via interaction with the CCR4-NOT complex. The YTHDF paralogs (YTHDF1, YTHDF2 and YTHDF3) shares m6A-containing mRNAs targets and act redundantly to mediate mRNA degradation and cellular differentiation. Required to facilitate learning and memory formation in the hippocampus by binding to m6A-containing neuronal mRNAs. Acts as a regulator of axon guidance by binding to m6A-containing ROBO3 transcripts. Acts as a negative regulator of antigen cross-presentation in myeloid dendritic cells. In the context of tumorigenesis, negative regulation of antigen cross-presentation limits the anti-tumor response by reducing efficiency of tumor-antigen cross-presentation. Promotes formation of phase-separated membraneless compartments, such as P-bodies or stress granules, by undergoing liquid-liquid phase separation upon binding to mRNAs containing multiple m6A-modified residues: polymethylated mRNAs act as a multivalent scaffold for the binding of YTHDF proteins, juxtaposing their disordered regions and thereby leading to phase separation. The resulting mRNA-YTHDF complexes then partition into different endogenous phase-separated membraneless compartments, such as P-bodies, stress granules or neuronal RNA granules.
• Product Name Alternative: C20orf21 antibody DACA 1 antibody DACA-1 antibody Dermatomyositis associated with cancer putative autoantigen 1 antibody YTH domain family 1 antibody YTH domain family member 1 antibody YTH domain family protein 1 antibody YTHD1 antibody YTHD1_HUMAN antibody Ythdf1 antibody
• Abbreviation: DF1 YTHDF1 DACA-1 C20orf21
• CAS Number: 9000-83-3
• Swiss Prot: Q9BYJ9 Human, P59326 Mouse,
• Cellular Locus: Cytoplasm, P-body.
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant protein within human YTHDF1 aa 300-559.
• Isotype: IgG
• Clone: PSH0-23
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IHC-P, FC
• Positive Control: HepG2 cell lysate, Hela cell lysate, 293T cell lysate, MCF-7 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, HCT116 cell lysate, mouse testis liver tissue lysate, rat testis tissue lysate, mouse brain tissue lysate, rat brain tissue lysate, human brain tissue, mouse brain tissue, mouse hippocampus tissue, rat brain tissue, 293, PC-12.
• Concentration: 1 mg/mL
• Dilution: WB: 1:1,000;IHC-P: 1:1,000;FC: 1ug/mL
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 61 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
• Recombinant Antibody: Yes