CD3E Rabbit Polyclonal Antibody

• Description:

  The CD3 protein is a T-cell marker, a complex of four structurally distinct membrane glycoprotein isoforms, 20-50 kDa, comprising extracellular, transmembrane and intracellular domains. The CD3 complex is responsible for mediating signal transduction to the internal environment upon antigenic recognition by TCR, causing T-cell proliferation and release of cytokines. Except for a weak expression in Purkinje cells (with some of the Abs) and activated NK-cells, CD3 is found only in T-cells. CD3 appear in the cytoplasm of prothymocytes, and on the surface of about 95% of thymocytes, while cytoplasmic CD3 is lost as the cells differentiate into medullary thymocytes. In therapy resistant celiac disease, a shift from membranous to cytoplasmic CD3 expression is seen (together with loss of CD8). In malignant lymphomas, CD3 is a pan-T-cell lineage-restricted antigen, detected in 80-97% of the T-cell lymphomas. Mature T-cell lymphomas including cases of mycosis fungoides, peripheral T-cell lymphoma and anaplastic large cell lymphoma may aberrantly lose CD3. NK-cell lymphomas can show a cytoplasmic reaction. Reed-Sternberg cells may show a globular paranuclear reaction. CD3 is an important marker in the classification of malignant lymphomas and lymphoid leukaemias. Also the marker is useful for the identification of T-cells in, e.g., celiac disease, lymphocytic colitis and colorectal carcinomas associated with loss of a mismatch repair protein.

• Product Name Alternative:

  CD3 epsilon antibody CD3e antibody CD3e antigen antibody CD3e antigen epsilon polypeptide (TiT3 complex) antibody CD3E antigen epsilon polypeptide antibody CD3E antigen, epsilon subunit antibody CD3e molecule epsilon antibody CD3e molecule, epsilon (CD3 TCR complex) antibody CD3e molecule, epsilon (CD3-TCR complex) antibody CD3E_HUMAN antibody IMD18 antibody T cell antigen receptor complex epsilon subunit of T3 antibody T cell surface antigen T3/Leu 4 epsilon chain antibody T cell surface glycoprotein CD3 epsilon chain antibody T-cell surface antigen T3/Leu-4 epsilon chain antibody T-cell surface glycoprotein CD3 epsilon chain antibody T3E antibody TCRE antibody

• Abbreviation:

  CD3E

• Swiss Prot:

  P07766 Human, P22646 Mouse,

• Cellular Locus:

  Cell membrane.

• Host:

  Rabbit

• Species Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthetic peptide within Human CD3E aa 158-207 / 207.

• Isotype:

  IgG

• Conjugation:

  Non-conjugated

• Type:

  Rabbit polyclonal Antibody

• Applications:

  WB

• Positive Control:

  Jurkat cell lysate, mouse thymus tissue lysate, rat thymus tissue lysate.

• Concentration:

  1 mg/mL

• Dilution:

  WB: 1:500

• Purity:

  Immunogen affinity purified.

• Form:

  Liquid

• Buffer:

  PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• Molecular Weight:

  Predicted band size: 23 kDa

• Storage Conditions:

  Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

• Recombinant Antibody:

  No

• CAS Number:

  9007-83-4