macroH2A.1 Recombinant Rabbit Monoclonal Antibody [JU22-83]

macroH2A.1 Recombinant Rabbit Monoclonal Antibody [JU22-83]

• Description: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation. Inhibits the binding of transcription factors and interferes with the activity of remodeling SWI/SNF complexes. Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces an hypoacetylated state of chromatin. In addition, isoform 1, but not isoform 2, binds ADP-ribose and O-acetyl-ADP-ribose, and may be involved in ADP-ribose-mediated chromatin modulation.
• Product Name Alternative: Core histone macro h2a.1 antibody Core histone macro-H2A.1 antibody H2A histone family member Y antibody H2A.y antibody H2A/y antibody H2AF12M antibody H2AFJ antibody H2afy antibody H2AY_HUMAN antibody Histone H2A.Y antibody Histone macroH2A1 antibody Histone macroH2A1.1 antibody Histone macroH2A1.2 antibody Macroh2a1 antibody MACROH2A1.1 antibody MacroH2A1.2 antibody Medulloblastoma antigen MU MB 50.205 antibody Medulloblastoma antigen MU-MB-50.205 antibody mH2a antibody mH2A1 antibody
• Abbreviation: Histone macroH2A1, mH2A1, H2A/y, H2AFY, MACROH2A1
• CAS Number: 9000-83-3
• Swiss Prot: O75367 Human, Q9QZQ8 Mouse, Q02874 Rat
• Cellular Locus: Nucleus.
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic peptide within Human macroH2A1 aa 232-281 / 369.
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IF-Cell, IF-Tissue, IHC-P
• Positive Control: Hela, HepG2, PC-3M, human lung cancer tissue using anti-macroH2A.1 antibody. Counter stained, human thyroid gland tissue, human placenta tissue, mouse fallopian tube tissue.
• Concentration: 1 mg/mL
• Dilution: WB: 1:500-1:2,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:50-1:200
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: 40 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Recombinant Antibody: Yes