Cdk4 Recombinant Rabbit Monoclonal Antibody [SD205-1]

Cdk4 Recombinant Rabbit Monoclonal Antibody [SD205-1]

• Description: The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16INK4a. This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb).Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
• Product Name Alternative: Cdk 4 antibody cdk4 antibody CDK4 protein antibody CDK4_HUMAN antibody Cell division kinase 4 antibody Cell division protein kinase 4 antibody CMM 3 antibody CMM3 antibody Crk3 antibody Cyclin dependent kinase 4 antibody Cyclin-dependent kinase 4 antibody Melanoma cutaneous malignant 3 antibody MGC14458 antibody p34 cdk4 antibody PSK J3 antibody PSK-J3 antibody
• Abbreviation: CDK4
• CAS Number: 9000-83-3
• Swiss Prot: P11802 Human, P30285 Mouse, P35426 Rat
• Cellular Locus: Cytoplasm, Nucleus, Membrane.
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant protein within mouse Cdk4 aa 180-303.
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IF-Cell, IF-Tissue, IHC-P, IP, FC
• Positive Control: SK-OV-3 cell lysate, Jurkat cell lysate, HeLa cell lysate, MCF7 cell lysate, K-562 cell lysate, A549 cell lysate, NIH:OVCAR-3 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, C6 cell lysate, PC-12 cell lysate, Mouse brain tissue lysate, Rat lung tissue lysate, human thyroid carcinoma tissue, mouse colon tissue, mouse lung tissue, rat colon tissue, rat lung tissue, NIH/3T3, HeLa.
• Concentration: 1 mg/mL
• Dilution: WB: 1:2,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:200-1:1,000 ;IP: Use at an assay dependent concentration. ;FC: 1:1,000
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 34 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Recombinant Antibody: Yes