Histone H2A (acetyl K9) Recombinant Rabbit Monoclonal Antibody [SR4-15]

Histone H2A (acetyl K9) Recombinant Rabbit Monoclonal Antibody [SR4-15]

• Description: Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.
• Product Name Alternative: H2A antibody H2A1B_HUMAN antibody H2AFM antibody HIST1H2A antibody HIST1H2AE antibody Histone H2A type 1-B/E antibody Histone H2A.2 antibody Histone H2A/a antibody Histone H2A/m antibody H2AK9ac antibody
• Abbreviation: HIST1H2AB, HIST1H2AC, HIST1H2AJ,H2AK9ac
• CAS Number: 9000-83-3
• Swiss Prot: P04908 Human,Q93077 Human, Q99878 Human, C0HKE6 Mouse, C0HKE1 Mouse, P02262 Rat
• Cellular Locus: Nucleus, Chromosome.
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic peptide within Human Histone H2A aa aa 1-50 / 130 (acetyl K9) conjugated to Keyhole Limpet Haemocyanin (KLH).
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IF-Cell, IF-Tissue, IHC-P, ChIP, CUT&Tag-seq
• Positive Control: HeLa treated with 500 ng/ml Trichostatin A for 4 hours whole cell lysates, A549, MCF-7, human tonsil tissue, human colon tissue, mouse testis tissue.
• Concentration: 1 mg/mL
• Dilution: WB: 1:500 ;IF-Cell: 1:50 ;IF-Tissue: 1:50 ;IHC-P: 1:50-1:1,000 ;ChIP: Use 0.5~2 μg for 25 μg of chromatin.
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 14 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Recombinant Antibody: Yes