Histone H2B (acetyl K20) Recombinant Rabbit Monoclonal Antibody [PS01-46]

Histone H2B (acetyl K20) Recombinant Rabbit Monoclonal Antibody [PS01-46]

• Description: Histone H2B is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and long N-terminal and C-terminal tails, H2B is involved with the structure of the nucleosomes. Histone H2B is a structural protein that helps organize eukaryotic DNA. It plays an important role in the biology of the nucleus where it is involved in the packaging and maintaining of chromosomes, regulation of transcription, and replication and repair of DNA. Histone H2B helps regulate chromatin structure and function through post-translational modifications and specialized histone variants. Acetylation and ubiquitination are examples of two post-translational modifications that affect the function of histone H2B in particular ways. Hyperacetylation of histone tails helps DNA-binding proteins access chromatin by weakening histone-DNA and nucleosome-nucleosome interactions. Furthermore, acetylation of a specific lysine residue binds to bromine-containing domains of certain transcription and chromatin regulatory proteins. This docking facilitates the recruitment of these proteins to the correct region of the chromosome. Ubiquitinated histone H2B is often found in regions of active transcription. Through the facilitation of chromatin remodeling, it stimulates transcriptional elongation and sets the stage for further modifications that regulate multiple elements of transcription.
• Product Name Alternative: GL105 antibody H2B GL105 antibody H2B histone family member O antibody H2B histone family member S antibody H2B.1 antibody H2B.1 B antibody H2B.b antibody H2B.c antibody H2B.d antibody H2B.e antibody H2B.f antibody H2B.j antibody H2B.q antibody H2B/b antibody H2B/c antibody H2B/d antibody H2B/e antibody H2B/f antibody H2B/j antibody H2B/o antibody H2B/q antibody H2BFB antibody H2BFC antibody H2BFD antibody H2BFE antibody H2BFF antibody H2BFJ antibody H2BFO antibody H2BFQ antibody H2BFS antibody H2BGL105 antibody H2BQ antibody HIRIP2 antibody HIST1H2BB antibody HIST1H2BD antibody HIST1H2BH antibody HIST1H2BL antibody HIST1H2BM antibody HIST1H2BN antibody HIST2H2BE antibody histone cluster 2 antibody Histone H2B antibody histone H2B GL105 antibody Histone H2B type 1 B antibody Histone H2B type 1 D antibody Histone H2B type 1 H antibody Histone H2B type 1 L antibody Histone H2B type 1 M antibody Histone H2B type 1 N antibody Histone H2B type 2 E antibody Histone H2B.q antibody histone protein antibody
• Abbreviation: H2BC12
• CAS Number: 9000-83-3
• Swiss Prot: O60814 Human, P06899 Human, P23527 Human, P33778 Human, P57053 Human, P58876 Human, P62807 Human, Q16778 Human, Q5QNW6 Human, Q8N257 Human, Q93079 Human, Q99877 Human, Q99879 Human, Q99880 Human, P10854 Mouse, Q64475 Mouse, Q00729 Rat
• Cellular Locus: Nucleus, Chromosome.
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic peptide.
• Isotype: IgG
• Clone: PS01-46
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IHC-P, IF-Cell, ChIP
• Positive Control: Hela treated with 500 ng/ml TSA for 4 hours whole cell lysate, human colon carcinoma tissue, human skin tissue, mouse skin tissue, rat skin tissue, Hela, NIH/3T3.
• Concentration: 1 mg/mL
• Dilution: WB: 1:1,000 ;IHC-P: 1:1,000 ;IF-Cell: 1:50 ;ChIP: Use 0.5~2 μg for 25 μg of chromatin.
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 14 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
• Recombinant Antibody: Yes