Histone H3 (acetyl K27) Rabbit Polyclonal Antibody

Histone H3 (acetyl K27) Rabbit Polyclonal Antibody

• Description: Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fibers. Two molecules of each of the four core histones (H2A, H2B, H3 and H4) form the octamer, which is comprised of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Human and mouse Histone H4 are subject to methylation at Lys 20, a modification that may be necessary for select DNA transactions or chromatin state transitions.
• Product Name Alternative: H3 histone family member E pseudogene antibody H3 histone family, member A antibody H3/A antibody H31_HUMAN antibody H3F3 antibody H3FA antibody Hist1h3a antibody HIST1H3B antibody HIST1H3C antibody HIST1H3D antibody HIST1H3E antibody HIST1H3F antibody HIST1H3G antibody HIST1H3H antibody HIST1H3I antibody HIST1H3J antibody HIST3H3 antibody histone 1, H3a antibody Histone cluster 1, H3a antibody Histone H3 3 pseudogene antibody Histone H3.1 antibody Histone H3/a antibody Histone H3/b antibody Histone H3/c antibody Histone H3/d antibody Histone H3/f antibody Histone H3/h antibody Histone H3/i antibody Histone H3/j antibody Histone H3/k antibody Histone H3/l antibody H3K27ac antibody
• Abbreviation: Histone H3 H3K27ac
• CAS Number: 9007-83-4
• Swiss Prot: P68431 Human, P84243 Human, Q16695 Human, Q71DI3 Human, Q6NXT2 Human
• Cellular Locus: Chromosome, Nucleosome core, Nucleus.
• Host: Rabbit
• Species Reactivity: Human, Mouse
• Immunogen: Synthetic peptide within human Histone H3 (acetyl K27) aa 1-50.
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Rabbit polyclonal Antibody
• Applications: WB, IHC-P, ChIP
• Positive Control: Hela treated with TSA whole cell lysate, NIH/3T3 treated with TSA whole cell lysate, Hela cell lysate treated with Sodium Butyrate 0.5mM for 24h, human skin tissue, mouse colon tissue, human colon tissue, human colon carcinoma tissue.
• Concentration: 1 mg/mL
• Dilution: WB: 1:500-1:2,000 ;IHC-P: 1:100-1:500 ;ChIP: Use 0.5~2 μg for 25 μg of chromatin.
• Purity: Immunogen affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 15 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
• Recombinant Antibody: No