Histone H2B (acetyl K20) Recombinant Rabbit Monoclonal Antibody [SN72-09]

Histone H2B (acetyl K20) Recombinant Rabbit Monoclonal Antibody [SN72-09]

• Description: Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.
• Product Name Alternative: H2B 1A antibody H2B antibody H2B histone family antibody H2B2f antibody H2B2F_HUMAN antibody H2Ba antibody H2Bf antibody HIST2H2BF antibody histone H2B antibody histone H2B type 1 antibody Histone H2B type 2-F antibody MGC131639 antibody H2BK20ac antibody
• Abbreviation: Hist1h2ba H2BK20ac
• CAS Number: 9000-83-3
• Swiss Prot: O60814 Human, P06899 Human, P23527 Human, P33778 Human, P57053 Human, P58876 Human, P62807 Human, Q16778 Human, Q5QNW6 Human, Q8N257 Human, Q93079 Human, Q99877 Human, Q99879 Human, Q99880 Human,
• Cellular Locus: Chromosome, Nucleosome core, Nucleus.
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic peptide within human Histone H2B aa 1-49/126.
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IF-Cell, IF-Tissue, IHC-P, FC, IP, ChIP, CUT&Tag-seq
• Positive Control: SW480, MCF-7, Hela, human tonsil tissue, human kidney tissue, human colon tissue, mouse colon tissue, human colon cancer tissue, mouse kidney tissue, human breast carcinoma tissue.
• Concentration: 1 mg/mL
• Dilution: WB: 1:1,000 ;IF-Cell: 1:100-1:500 ;IF-Tissue: 1:100-1:500 ;IHC-P: 1:100-1:500 ;FC: 1:50-1:100 ;IP: Use at an assay dependent concentration. ;ChIP: Use 0.5~2 μg for 25 μg of chromatin.
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 14 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Recombinant Antibody: Yes