Phospho-Histone H2A.X (S139) Recombinant Rabbit Monoclonal Antibody [SR33-09]

Phospho-Histone H2A.X (S139) Recombinant Rabbit Monoclonal Antibody [SR33-09]

• Description: Histone H2A.X is a member of the Histone H2A family, which is involved in nucleosomal organization of chromatin. An important phosphorylated form is γH2AX (S139), which forms when double-strand breaks appear. In humans and other eukaryotes, the DNA is wrapped around histone octamers, consisting of core histones H2A, H2B, H3 and H4, to form chromatin. H2AX contributes to nucleosome-formation, chromatin-remodeling and DNA repair, and is also used in vitro as an assay for double-strand breaks in dsDNA.
• Product Name Alternative: AW228881 antibody H2A histone family member X antibody H2A.FX antibody H2A.X antibody H2a/x antibody H2AFX antibody H2AX antibody H2AX histone antibody H2AX_HUMAN antibody Hist5.2ax antibody Histone 2A antibody Histone 2AX antibody Histone H2A.X antibody Histone H2AX antibody RGD1566119 antibody gamma H2A.X antibody γ H2A.X antibody γ-H2A.X antibody
• Abbreviation: H2a/x, gamma H2A.X, H2AX,γ H2A.X,γ-H2A.X
• CAS Number: 9000-83-3
• Swiss Prot: P16104 Human, P27661 Mouse
• Cellular Locus: Nucleus, Chromosome
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser139 of Human Histone H2AX.
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IHC-P
• Positive Control: HeLa treated with 20μM Etoposide for 2 hours whole cell lysate, HeLa treated with UV for 2 hours whole cell lysate, NIH/3T3 treated with 25μM Etoposide for 5 hours whole cell lysate, HepG2 cell lysate–treated with etoposide, rat brain tissue, mouse testis tissue, mouse brain tissue, mouse small intestine tissue, human colon carcinoma tissue.
• Concentration: 1 mg/mL
• Dilution: WB: 1:1,000-1:2,000 ;IHC-P: 1:50-1:5000
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 15 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Recombinant Antibody: Yes