Phospho-Histone H1.3 (T17)+Histone H1.4 (T17) Recombinant Rabbit Monoclonal Antibody [SR38-03]

Phospho-Histone H1.3 (T17)+Histone H1.4 (T17) Recombinant Rabbit Monoclonal Antibody [SR38-03]

• Description: Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.
• Product Name Alternative: Histone H1.3 Histone H1c Histone H1s-2 HIST1H1DH1F3 Histone H1.4 Histone H1b Histone H1s-4 HIST1H1EH1F4
• Abbreviation: HIST1H1D, HIST1H1E
• CAS Number: 9000-83-3
• Swiss Prot: P10412 Human, P16402 Human, P43274 Mouse, P43277 Mouse,
• Cellular Locus: Nucleus, Chromosome.
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Thr17 of Human H14.
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IF-Cell, IF-Tissue, IHC-P
• Positive Control: CRC cell lysate, NIH/3T3, CRC, human colon carcinoma tissue, human skin tissue, human breast carcinoma tissue.
• Concentration: 1 mg/mL
• Dilution: WB: 1:500 ;IF-Cell: 1:50 ;IF-Tissue: 1:50 ;IHC-P: 1:200
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 22 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Recombinant Antibody: Yes