Phospho-Histone H3 (S10) Recombinant Rabbit Monoclonal Antibody [SA31-01]

Phospho-Histone H3 (S10) Recombinant Rabbit Monoclonal Antibody [SA31-01]

• Description: Histone H3 is one of the five main histones involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Histone proteins are highly post-translationally modified however Histone H3 is the most extensively modified of the five histones. The term "Histone H3" alone is purposely ambiguous in that it does not distinguish between sequence variants or modification state. Histone H3 is an important protein in the emerging field of epigenetics, where its sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes.
• Product Name Alternative: H3 3 like sequence MH921 antibody H3 3A antibody H3 a antibody H3 b antibody H3 c antibody H3 d antibody H3 f antibody H3 h antibody H3 histone family member E pseudogene antibody H3 i antibody H3 j antibody H3 k antibody H3 l antibody H33_HUMAN antibody H3F3 antibody H3f3b antibody Histone H3 3 pseudogene antibody Histone H3.3 antibody
• Abbreviation: Histone H3.3
• CAS Number: 9000-83-3
• Swiss Prot: P68431 Human, P84243 Human, Q16695 Human, Q6NXT2 Human, Q71DI3 Human, P84244 Mouse, P84245 Rat
• Cellular Locus: Nucleus, Chromosome
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser10 of human Histone H3.
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IF-Cell, IF-Tissue, IHC-P, IP, FC, ChIP
• Positive Control: HeLa treated with 50nM Calyculin A for 30 minutes whole cell lysate, NIH/3T3 treated with 100nM Calyculin A for 30 minutes whole cell lysate, C6 treated with 100nM Calyculin A for 30 minutes whole cell lysate, HeLa, human kidney tissue, mouse kidney tissue, rat kidney tissue.
• Concentration: 1 mg/mL
• Dilution: WB: 1:2,000-1:10,000 ;IF-Cell: 1:5,000 ;IF-Tissue: 1:50 ;IHC-P: 1:100 ;IP: Use at an assay dependent concentration. ;FC: 1:1,000 ;ChIP: Use 0.5~2 μg for 25 μg of chromatin.
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 15 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Recombinant Antibody: Yes