Phospho-Histone H3 (S28) Recombinant Rabbit Monoclonal Antibody [JE56-06]

Phospho-Histone H3 (S28) Recombinant Rabbit Monoclonal Antibody [JE56-06]

• Description: Histone H3 is one of the five main histones involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Histone proteins are highly post-translationally modified however Histone H3 is the most extensively modified of the five histones. The term "Histone H3" alone is purposely ambiguous in that it does not distinguish between sequence variants or modification state. Histone H3 is an important protein in the emerging field of epigenetics, where its sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes.
• Product Name Alternative: H3 histone family 3A antibody H3 histone family 3B antibody H3 histone, family 3B (H3.3B) antibody H3.3 antibody H3.3A antibody H3.3B antibody H33_HUMAN antibody H3F3 antibody H3F3A antibody H3f3b antibody Histone H3.3 antibody Histone H3.3Q antibody Histone H3.A antibody Histone H3.B antibody MGC87782 antibody MGC87783 antibody
• Abbreviation: H3-3A, H3-3B
• CAS Number: 9000-83-3
• Swiss Prot: P68431 Human, P68433 Mouse, Q6LED0 Rat
• Cellular Locus: Nucleus, Chromosome
• Host: Rabbit
• Species Reactivity: Human, Rat, Mouse
• Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Ser28 of human Histone H3.
• Isotype: IgG
• Clone: JE56-06
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IF-Cell, IHC-P, FC, ChIP
• Positive Control: HeLa treated with 100ng/mL Nocodazole for 18 hours cell lysate, PC-12 treated with 100ng/mL Nocodazole for 18 hours cell lysate, HeLa, rat colon tissue.
• Concentration: 1 mg/mL
• Dilution: WB: 1:1,000 ;IF-Cell: 1:10,000 ;IHC-P: 1:2,000 ;FC: 1:1,000 ;ChIP: Use 0.5~2 μg for 25 μg of chromatin.
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 15 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Recombinant Antibody: Yes