Histone H1.0 Recombinant Rabbit Monoclonal Antibody [SD206-04]

Histone H1.0 Recombinant Rabbit Monoclonal Antibody [SD206-04]

• Description: Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.
• Product Name Alternative: H1 histone family member 0 antibody H1 (0) antibody H10 antibody H10_HUMAN antibody h1f0 antibody H1FV antibody Histone H1' antibody Histone H1 (0) antibody Histone H1.0 antibody Histone H10 antibody Histone H5 antibody MGC5241 antibody N-terminally processed antibody
• Abbreviation: H1F0
• CAS Number: 9000-83-3
• Swiss Prot: P07305 Human, P10922 Mouse, P43278 Rat
• Cellular Locus: Nucleus, Chromosome.
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic peptide within Human Histone H10 aa 1-50 / 194.
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IF-Cell, IF-Tissue, IHC-P
• Positive Control: NIH/3T3 cell lysate, PC-3 cell lysate, NIH/3T3, human tonsil tissue, human colon carcinoma tissue, human pancreas tissue, mouse colon tissue, human kidney tissue, mouse liver tissue.
• Concentration: 1 mg/mL
• Dilution: WB: 1:500-1:2,000;IF-Cell: 1:50-1:100;IF-Tissue: 1:50-1:100;IHC-P: 1:50-1:800
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 21 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Recombinant Antibody: Yes