MCM7 Rabbit Polyclonal Antibody

• Description:

  Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage. Early fractionation of eukaryotic MCM proteins yielded a variety of dimeric, trimeric and tetrameric complexes with unclear biological significance. Specifically a MCM467 subcomplex is shown to have in vitro helicase activity which is inhibited by the MCM2 subunit. The MCM2-7 hexamer is the proposed physiological active complex.

• Product Name Alternative:

  CDABP0042 antibody CDC 47 antibody CDC47 antibody CDC47 homolog antibody Cdc47, S. cerevisiae, homolog of antibody DNA replication licensing factor MCM7 antibody Homolog of S. cerevisiae Cdc47 antibody MCM 2 antibody MCM 7 antibody MCM2 antibody MCM2, formerly antibody Mcm7 antibody MCM7 minichromosome maintenance deficient 7 antibody MCM7_HUMAN antibody Minichromosome Maintainence 7 antibody Minichromosome maintainence, S. cerevisiae, homolog of antibody Minichromosome maintenance complex component 7 antibody Minichromosome maintenance deficient 7 antibody Minichromosome maintenance protein 7 antibody P1.1 MCM3 antibody P1.1-MCM3 antibody P1CDC47 antibody P85MCM antibody PNAS 146 antibody PNAS146 antibody PPP1R104 antibody Protein phosphatase 1, regulatory subunit 104 antibody

• Abbreviation:

  DNA replication licensing factor MCM7 , CDC47 homolog,P1.1-MCM3,MCM7

• Swiss Prot:

  P33993 Human, Q61881 Mouse, Q6AYN8 Rat

• Cellular Locus:

  Nucleus.

• Host:

  Rabbit

• Species Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant protein corresponding to C terminal Human MCM7.

• Isotype:

  IgG

• Conjugation:

  Non-conjugated

• Type:

  Rabbit polyclonal Antibody

• Applications:

  WB, IHC-P, IF-Cell, FC

• Positive Control:

  MCF-7 cell lysate, K562 cell lysate, mouse colon tissue lysate, MCF-7, MG-63, SHSY5Y, human lung cancer tissue, human colon tissue, mouse skin tissue.

• Concentration:

  1 mg/mL

• Dilution:

  WB: 1:500-1:2,000;IHC-P: 1:50-1:200;IF-Cell: 1:50-1:200;FC: 1:50-1:100

• Purity:

  Immunogen affinity purified.

• Form:

  Liquid

• Buffer:

  1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

• Molecular Weight:

  Predicted band size 81/60/45 kDa.

• Storage Conditions:

  Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

• Recombinant Antibody:

  No

• CAS Number:

  9007-83-4