Biotinylated SARS-CoV-2 Spike RBD Protein, His, Avitag™ (EG.5/Omicron) (MALS verified)

• Background:

  It's been reported that coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

• Description:

  Biotinylated SARS-CoV-2 Spike RBD Protein, His, Avitag (EG.5/Omicron) (SPD-C82Q5) is expressed from human 293 cells (HEK293) . It contains AA Arg 319 - Lys 537 (Accession # QHD43416.1 (G339H, R346T, L368I, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, V445P, G446S, F456L, N460K, S477N, T478K, E484A, F486P, F490S, Q498R, N501Y, Y505H) ) . The spike mutations are identified on the SARS-CoV-2 Omicron variant (Pango lineage: EG.5) .

• Specifications:

  This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 28.2 kDa. The protein migrates as 36-41 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

• Accession Number:

  QHD43416.1 (G339H, R346T, L368I, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, V445P, G446S, F456L, N460K, S477N, T478K, E484A, F486P, F490S, Q498R, N501Y, Y505H)

• Expression Region:

  Arg 319 - Lys 537

• Host:

  HEK293

• Target:

  Spike RBD

• Conjugation:

  Biotin-labeled

• Tag:

  C-10xHis & C-Avi

• Source:

  SARS-CoV-2

• Stability:

  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Endotoxin:

  1.0 EU per μg

• Purity:

  90%

• Bioactivity:

  Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 5 μg/mL (100 μL/well) can bind Biotinylated SARS-CoV-2 Spike RBD Protein, His,Avitag (EG.5/Omicron) (Cat. No. SPD-C82Q5) with a linear range of 1.953-31.25 ng/mL (QC tested).

• Format:

  Powder

• Buffer:

  PBS, pH7.4

• Additives:

  Trehalose

• Molecular Weight:

  28.2 kDa

• Additionnal Information:

  Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments

• Shipping Conditions:

  RT

• Storage Conditions:

  -20°C

• Package Size:

  25ug*1

• Host or Source:

  HEK293

• Species:

  SARS-CoV-2

• Protein ID:

  QHD43416.1 (G339H, R346T, L368I, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, V445P, G446S, F456L, N460K, S477N, T478K, E484A, F486P, F490S, Q498R, N501Y, Y505H)

• Preservative:

  Trehalose