SARS-CoV-2 Spike S2 Protein, His Tag

• Background:

  It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

• Description:

  SARS-CoV-2 Spike S2, His Tag (S2N-C52H5) is expressed from human 293 cells (HEK293) . It contains AA Ser 686 - Pro 1213 (Accession # QHD43416.1) . Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) are introduced to prevent the formation of aggregates in the course of protein production.

• Specifications:

  This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 60.0 kDa. The protein migrates as 65-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• Accession Number:

  QHD43416.1

• Expression Region:

  Ser 686 - Pro 1213

• Host:

  HEK293

• Target:

  Spike S2

• Conjugation:

  Unconjugated

• Tag:

  C-10xHis

• Source:

  SARS-CoV-2

• Stability:

  ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Endotoxin:

  1.0 EU per μg

• Purity:

  90%

• Bioactivity:

  Immobilized SARS-CoV-2 Spike S2, His Tag (Cat. No. S2N-C52H5) at 1 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike S2 Antibody, Human IgG1 with a linear range of 0.4-3 ng/mL (QC tested).

• Format:

  Powder

• Buffer:

  PBS, pH7.4

• Additives:

  Trehalose

• Molecular Weight:

  60.0 kDa

• Shipping Conditions:

  RT

• Storage Conditions:

  -20°C

• Package Size:

  100ug*1

• Species:

  SARS-CoV-2