Integrin alpha 3 Antibody / ITGA3

• Description:

  ITGA3 (INTEGRIN, ALPHA-3), also called CD49C, VLA3 or GAPB3, is a protein that in humans is encoded by the ITGA3 gene. It is an integrin alpha subunit which is also a member of the family of cell surface adhesion molecules. This gene is mapped to chromosome 17 and its exact cytogenetic location is 17q21.33. ITGA3 makes up half of the ?3?1 integrin duplex that plays a role in neural migration and corticogenesis together with beta-1 subunit. A functional link between DAB1 phosphorylation and ITGA3 signaling drives the timely detachment of migrating neurons from radial glial fibers. Expression of human ITGA3 increased the infectivity of virus for Chinese hamster ovary cells. ITGA3 also contains 13 potential N-glycosylation sites, 2 potential cleavage sites, and the 7 N-terminal repeating units characteristic of ITGAs. Recombinant ITGA3 is expressed as a 150-kD protein as the same size as the native protein by the western blot analysis.

• Specifications:

  Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

• UniProt:

  P26006

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Recombinant human protein (amino acids L96-L324) was used as the immunogen for the Integrin alpha 3 antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, FACS, IHC-P, IF, Direct ELISA

• Purity:

  Antigen affinity purified

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose

• Reconstitution:

  After reconstitution, the Integrin alpha 3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This Integrin alpha 3 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the Integrin alpha 3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the Integrin alpha 3 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Image Legend:

  IHC staining of FFPE human lung adenocarcinoma tissue with Integrin alpha 3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.