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ITGA4 Antibody / Integrin Alpha 4

CAT: 0800-FY12794Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-FY12794Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Specifications
Western Blot: 0.25-0.5 µg/mL, ELISA: 0.1-0.5 µg/mL
UniProt
P13612
Host
Rabbit
Reactivity
Human
Immunogen
E.coli-derived human Integrin Alpha 4/ITGA4 recombinant protein (Position: N35-V269) was used as the immunogen for the ITGA4 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, ELISA
Purity
Immunogen affinity purified
Format
Lyophilized
Buffer
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Limitations
This ITGA4 antibody is available for research use only.
Storage Conditions
After reconstitution, the ITGA4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml
Applications Notes
Optimal dilution of the ITGA4 antibody should be determined by the researcher.
Image Legend
Western blot analysis of Integrin Alpha 4/ITGA4 using anti-ITGA4 antibody. Lane 1: human Jurkat whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: human U87 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITGA4 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. A broad band is detected at ~150 kDa in most samples, consistent with the mature glycosylated alpha 4 subunit; U87 shows a prominent ~130-140 kDa band with a minor lower species, consistent with cell-type-dependent glycosylation and limited processing relative to the ~115 kDa core mass.

UniProtKB · P13612

Integrin alpha-4

ITA4_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P13612
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
ITGA4
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
D3DPG4, Q7Z4L6
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Ligand

CalciumMetal-binding

Biological process

Cell adhesion

PTM

Disulfide bondGlycoproteinPhosphoprotein

Molecular function

IntegrinReceptor

Cellular component

Membrane

Domain

RepeatSignalTransmembraneTransmembrane helix

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