CBX1, GST-tag Recombinant

• Background:

  Component of heterochromatin. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The protein may play an important role in the epigenetic control of chromatin structure and gene expression. Recognizes and binds histone H3 tails methylated at Lys9, leading to epigenetic repression. Interaction with lamin B receptor (LBR) can contribute to the association of the heterochromatin with the inner nuclear membrane.

• Description:

  Human chromobox homolog 1 (CBX1), also known as p25beta, M31, HP1-beta, and MOD1, GenBank Accession No. NM_006807, a.a. 2-185 (end), with N-terminal GST-tag, MW=48.1 kDa, expressed in anE. colicell expression system.

• UniProt:

  P83916

• Tag:

  N-terminal GST-tag

• Applications:

  Useful for protein binding assays, screening inhibitors, and selectivity profiling.

• Purity:

  ≥ 78%

• Format:

  Aqueous buffer solution

• Shipping Conditions:

  -80°C

• Storage Conditions:

  ≥ 6 months at -80°C

• Calculated Molecular Weight:

  48.1 kDa

• Formulation:

  40 mM Tris-HCl, pH 8.0,110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, and 20% glycerol.