HDAC4, GST-Tag Recombinant

• Background:

  HDAC4, or histone deacetylate 4, is a Class II member of the histone deacetylase family which is involved in lysine deacetylation. Lysine acetylation/deacetylation is a dynamic process involved in the regulation of a variety of cellular functions, similarly to phosphorylation/dephosphorylation. It interacts with MEF2C (myocyte-specific enhancer factor 2c) and MEF2D and this interaction allows regulation of gene expression. It also functions in multimeric protein complexes. HDAC4 is regulated by phosphorylation, SUMOylation and proteolytic cleavage, which influence the protein localization in the cell. It is involved in bone and muscle development, and vision. Dysfunction of HDAC4 seems to link to cancer in a context-dependent mode, but the mechanisms behind it are still unclear. It has been linked to multiple myeloma (MM) and acute myeloid leukemia (AML) . The development of new inhibitors specifically targeting HDAC4, in a disease-specific context, and a better understanding of its modes of action may open newer avenues of HDAC4-linked diseases.

• Description:

  Recombinant human HDAC4 (histone deacetylate 4) encompassing amino acids 101-end. This construct contains a C-terminal GST-tag. The recombinant protein was affinity purified and is active.

• UniProt:

  P56524

• Tag:

  C-terminal GST-tag

• Applications:

  Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

• Purity:

  ≥ 70% (Purity calculation does not include co-purifying Glutathione-binding proteins.)

• Format:

  Aqueous buffer solution

• Shipping Conditions:

  -80°C

• Storage Conditions:

  At least 6 months at -80°C.

• Calculated Molecular Weight:

  155 kDa

• Formulation:

  50 mM Tris-HCl, pH 7.5,150 mM NaCl, 10 mM Glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol