Recombinant CD33 Antibody

• Description:

  Recognizes a 67kDa glycoprotein, which is identified as CD33. It is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM) -containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies; these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia. CD33 is expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells. It is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells.

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL

• UniProt:

  P20138

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 51-224 was used as the immunogen for the recombinant CD33 antibody.

• Clonality:

  Recombinant Monoclonal

• Isotype:

  IgG κ

• Clone:

  SIGLEC3/7046R

• Type:

  Recombinant

• Applications:

  IHC-P

• Purity:

  Protein A/G affinity

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Aliquot the recombinant CD33 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This recombinant CD33 antibody is available for research use only.

• Storage Conditions:

  Aliquot the recombinant CD33 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the recombinant CD33 antibody should be determined by the researcher.

• CAS Number:

  9000-83-3

• Location:

  Cell surface

• Image Legend:

  SDS-PAGE analysis of purified, BSA-free recombinant CD33 antibody (clone SIGLEC3/7046R) as confirmation of integrity and purity.