CD33 Antibody

• Description :

  Recognizes a 67kDa glycoprotein, which is identified as CD33. It is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM) -containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies; these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia. CD33 is expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells. It is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells.

• Specifications :

  Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

• UniProt :

  P20138

• Host :

  Mouse

• Reactivity :

  Human

• Immunogen :

  A portion of amino acids 51-224 from the human protein was used as the immunogen for the CD33 antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG2c κ

• Clone :

  SIGLEC3/3600

• Applications :

  IHC-P

• Purity :

  Protein G affinity chromatography

• Format :

  Purified

• Buffer :

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution :

  Store the CD33 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations :

  This CD33 antibody is available for research use only.

• Storage Conditions :

  Store the CD33 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation :

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes :

  Optimal dilution of the CD33 antibody should be determined by the researcher.

• CAS Number :

  9007-83-4

• Location :

  Cell surface, cytoplasmic

• Image Legend :

  IHC staining of FFPE human urothelial carcinoma with CD33 antibody (clone SIGLEC3/3600) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.