CD33 Antibody

• Description:

  CD33 or Siglec-3 is a transmembrane receptor expressed on cells of myeloid lineage. It is usually considered myeloid-specific, but it can also be found on some lymphoid cells. It binds sialic acids, therefore is a member of the SIGLEC family of lectins. [Wiki]

• Specifications:

  Immunofluorescence: 1:10-1:50, Western blot: 1:100-1:500

• UniProt:

  P20138

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 337-364 from the human protein was used as the immunogen for this CD33 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgM

• Clone:

  574CT16.4.4

• Applications:

  WB, IF, ELISA

• Purity:

  Purified

• Format:

  Purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the CD33 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This CD33 antibody is available for research use only.

• Storage Conditions:

  Aliquot the CD33 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  Titration of the CD33 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  Fluorescent image of A549 cell stained with CD33 antibody at 1:25. CD33 immunoreactivity is localized to the membrane.