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Recombinant Synaptophysin Antibody

CAT: 0800-V8644-20UGSize: 20 µgDry Ice: NoHazardous: No
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CAT#:0800-V8644-20UGSize:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This monoclonal antibody recognizes a protein of 38kDa that is identified as synaptophysin. It is an N-glycosylated integral membrane protein found in neurons and endocrine cells. Synaptophysin contains four transmembrane domains and may function as a gap junction-like channel. This antibody identifies normal neuroendocrine cells and neuroendocrine neoplasms. Diffuse, finely granular, cytoplasmic staining is observed, which probably correlates with the distribution of the antigen within neurosecretory vesicles. Synaptophysin is an independent, broad-range marker of neural and neuroendocrine differentiation.
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT
UniProt
IDP08247
Host
Mouse
Reactivity
Human
Immunogen
A portion of amino acids 224-313 from the human protein was used as the immunogen for the recombinant Synaptophysin antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG2a κ
Clone
RSYP/4654
Type
Recombinant
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the recombinant Synaptophysin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This recombinant Synaptophysin antibody is available for research use only.
Storage Conditions
Store the recombinant Synaptophysin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the recombinant Synaptophysin antibody should be determined by the researcher.
Location
Cytoplasmic
Image Legend
IHC staining of FFPE human pancreas with recombinant Synaptophysin antibody (clone rSYP/4654) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

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