Synaptophysin Antibody

• Description:

  Synaptophysin (SYP) is an integral membrane protein of small synaptic vesicles in brain and endocrine cells. Ozcelik et al. (1990) concluded that the gene has 7 exons distributed over about 20 kb. The SYP locus mapped to Xp11.23-p11.22. Regionalization of the gene on the X chromosome was also done with hamster/human hybrid cells in which various portions of the human X chromosome were present.

• Specifications:

  Western blot: 0.5-1 µg/mL, IHC (FFPE) : 1-2 µg/mL, IHC (Frozen) : 1-2 µg/mL, formalin or acetone fixed tissues

• Host:

  Mouse

• Reactivity:

  Human, Rat

• Immunogen:

  Rat retina synaptosome was used as the immunogen for this Synaptophysin antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1

• Clone:

  SVP-38

• Applications:

  WB, IHC-P, IHC-F

• Purity:

  Unpurified ascites

• Format:

  Ascites

• Buffer:

  0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS

• Reconstitution:

  After reconstitution, the Synaptophysin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This Synaptophysin antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the Synaptophysin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1X PBS

• Applications Notes:

  The stated application concentrations are suggested starting points. Titration of the Synaptophysin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic, membranous

• Image Legend:

  Western blot testing of Synaptophysin antibody and rat brain tissue lysate. Predicted molecular weight: 34-38 kDa.