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Recombinant dsDNA Antibody

CAT: 0800-V8639-20UGSize: 20 µgDry Ice: NoHazardous: No
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CAT#:0800-V8639-20UGSize:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This MAb recognizes the double stranded DNA in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in human cells. This MAb produces a homogeneous staining pattern in the nucleus of normal and malignant cells. Double Stranded deoxyribonucleic acid (ds DNA) is the genetic material of all cells and many viruses and is a polymer of nucleotides. The monomer consists of phosphorylated 2-deoxyribose N-glycosidically linked to one of four bases, adenine, cytosine, guanine or thymine. These are linked together by 3-phosphodiester bridges. In the Watson-Crick double-helix model, two complementary strands are wound in a right-handed helix and held together by hydrogen bonds between complementary base pairs.
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Immunofluorescence: 1-3 µg/mL
UniProt
Not Applicable
Host
Mouse
Reactivity
Human
Immunogen
Nuclei of Burkitt cells were used as the immunogen for the recombinant dsDNA antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG2a κ
Clone
RDSD/4565
Type
Recombinant
Applications
IHC-P, IF
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the recombinant dsDNA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This recombinant dsDNA antibody is available for research use only.
Storage Conditions
Store the recombinant dsDNA antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the recombinant dsDNA antibody should be determined by the researcher.
Location
Nuclear
Image Legend
IHC staining of FFPE human colon with recombinant dsDNA antibody (clone rDSD/4565) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

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