DsDNA Antibody

CAT: 0800-V3099-100UGSize: 100 µgDry Ice: NoHazardous: No
CAT#:0800-V3099-100UGSize:100 µg
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PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This mAb recognizes the double stranded DNA in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in human cells. This mAb produces a homogeneous staining pattern in the nucleus of normal and malignant cells. Double Stranded deoxyribonucleic acid (ds DNA) is the genetic material of all cells and many viruses and is a polymer of nucleotides. The monomer consists of phosphorylated 2-deoxyribose N-glycosidically linked to one of four bases, adenine, cytosine, guanine or thymine. These are linked together by 3',5'-phosphodiester bridges. In the Watson-Crick double-helix model, two complementary strands are wound in a right-handed helix and held together by hydrogen bonds between complementary base pairs.
CAS Number
9007-83-4
Specifications
Flow Cytometry: 1-2ug/million cells in 0.1ml, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT (1)
UniProt
Not Known
Host
Mouse
Reactivity
Human
Immunogen
Nuclei of Burkitt's cells were used as the immunogen for the dsDNA antibody.
Clonality
Monoclonal
Isotype
IgG3 κ
Clone
AE-2
Applications
FACS, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the dsDNA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This dsDNA antibody is available for research use only.
Storage Conditions
Store the dsDNA antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the dsDNA antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Location
Nuclear
Image Legend
IHC staining of FFPE human colon carcinoma with dsDNA antibody (clone AE-2) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
Product Datasheet
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