DsDNA Antibody

• Description :

  This mAb recognizes the double stranded DNA in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in human cells. This mAb produces a homogeneous staining pattern in the nucleus of normal and malignant cells. Double Stranded deoxyribonucleic acid (ds DNA) is the genetic material of all cells and many viruses and is a polymer of nucleotides. The monomer consists of phosphorylated 2-deoxyribose N-glycosidically linked to one of four bases, adenine, cytosine, guanine or thymine. These are linked together by 3',5'-phosphodiester bridges. In the Watson-Crick double-helix model, two complementary strands are wound in a right-handed helix and held together by hydrogen bonds between complementary base pairs.

• Specifications :

  Flow Cytometry: 1-2ug/million cells in 0.1ml, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT (1)

• UniProt :

  Not Known

• Host :

  Mouse

• Reactivity :

  Human

• Immunogen :

  Nuclei of Burkitt's cells were used as the immunogen for the dsDNA antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG3 κ

• Clone :

  AE-2

• Applications :

  FACS, IHC-P

• Purity :

  Protein G affinity chromatography

• Format :

  Purified

• Buffer :

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution :

  Store the dsDNA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations :

  This dsDNA antibody is available for research use only.

• Storage Conditions :

  Store the dsDNA antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation :

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes :

  Optimal dilution of the dsDNA antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

• CAS Number :

  9007-83-4

• Location :

  Nuclear

• Image Legend :

  IHC staining of FFPE human colon carcinoma with dsDNA antibody (clone AE-2) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.