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DsDNA Antibody

CAT: 0800-V3075SAF-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V3075SAF-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Marker antibodies are useful in identification of organelles in cells, tissues, and biochemical preparations. This mAb recognizes the double stranded DNA in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in human cells. Double stranded (ds) DNA markers are useful tools in biology research and aid in the study of DNA behavior and characteristics.
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/million cells, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
Not Known
Host
Mouse
Reactivity
Human
Immunogen
Nuclei of Burkitt's cells were used as the immunogen for the dsDNA antibody.
Clonality
Monoclonal
Isotype
IgG3 κ
Clone
121-3
Applications
FACS, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Store the dsDNA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This dsDNA antibody is available for research use only.
Storage Conditions
Store the dsDNA antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
Optimal dilution of the dsDNA antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Location
Nuclear
Image Legend
IHC staining of FFPE human colon carcinoma with dsDNA antibody (clone 121-3) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
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