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MSLN Antibody / Mesothelin

CAT: 0800-V3821-20UGSize: 20 µgDry Ice: NoHazardous: No
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CAT#:0800-V3821-20UGSize:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Mesothelin is a 40kDa glycosyl-phosphatidylinositol-anchored glycoprotein cleaved from a 69kDa precursor protein. Mesothelin immunoreactivity is high in cancers of the ovary (serous papillary, endometrioid and undifferentiated) and pancreas, with less frequent staining seen in adenocarcinomas of the endometrium, lung and stomach/esophagus. Mesothelin is one of the most sensitive markers for mesothelioma.
CAS Number
9007-83-4
Specifications
IHC (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
Q13421
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
A portion of amino acids 273-407 from the human protein was used as the immunogen for this MSLN antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
MKPF-1
Applications
IHC-P
Purity
Protein G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
After reconstitution, the MSLN antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This MSLN antibody is available for research use only.
Storage Conditions
After reconstitution, the MSLN antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the MSLN antibody should be determined by the researcher.
Location
Cytoplasmic, cell surface, secreted
Image Legend
IHC testing of FFPE human lung mesothelioma with MSLN antibody (clone MKPF-1) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

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