TIMP2 Antibody

• Description :

  It recognizes a protein of 21kDa, identified as tissue inhibitor of metalloproteinases 2 (TIMP2) . It is closely related to TIMP1 and shows the highest binding affinity to both the latent (pro) and active forms of 72kDa Type IV collagenase (also known as MMP2 or gelatinase A) . It also has affinity for the active form of 92kDa Type IV collagenase (also known as MMP9 or gelatinase B) . TIMP2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.

• CAS Number :

  9007-83-4

• Specifications :

  Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

• UniProt :

  P16035

• Host :

  Mouse

• Reactivity :

  Human

• Immunogen :

  An amino acid sequence from the N-terminal region of the human protein was used as the immunogen for this TIMP2 antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG2a κ

• Clone :

  SPM356

• Applications :

  IHC-P

• Purity :

  Protein G affinity chromatography

• Format :

  Purified

• Buffer :

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution :

  Store the TIMP2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations :

  This TIMP2 antibody is available for research use only.

• Storage Conditions :

  Store the TIMP2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation :

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes :

  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TIMP2 antibody to be titered up or down for optimal performance.

• Location :

  Cytoplasmic, cell surface, secreted

• Image Legend :

  IHC testing of FFPE human placenta with TIMP2 antibody (clone SPM356) . Required HIER: requires boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.