TIMP4 Antibody

• Description:

  Metalloproteinase inhibitor 4 is an enzyme that in humans is encoded by the TIMP4 gene. And this gene is mapped to chromosome 3p25. This gene belongs to the TIMP gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. The secreted, netrin domain-containing protein encoded by this gene is involved in regulation of platelet aggregation and recruitment and may play role in hormonal regulation and endometrial tissue remodeling.

• Specifications:

  Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Flow cytometry: 1-3ug/10^6 cells, Immunofluorescence (FFPE) : 2-4 µg/mL, ELISA: 0.1-0.5 µg/mL (human protein tested; request BSA-free format for coating)

• UniProt:

  Q99727

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Amino acids N174-P224 from the human protein were used as the immunogen for the TIMP4 antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, IHC-P, FACS, IF, ELISA

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide

• Reconstitution:

  After reconstitution, the TIMP4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This TIMP4 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the TIMP4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the TIMP4 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Secreted

• Image Legend:

  IHC testing of FFPE human lung cancer tissue with TIMP4 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.