TIMP1 Antibody

• Description:

  TIMP-1, TIMP-2, TIMP-3 and TIMP-4 (for tissue inhibitor of metalloproteinases -1, -2, -3 and -4) complex with metalloproteinases such as collagenases, gelatinases and stromelysins, resulting in irreversible inactivation of the metalloproteinase. TIMP-1 is identical to EPA (erythroid-potentiation activity) . PTH has been shown to be a regulator of TIMP-2 in osteoblastic cells. TIMP-3 may be involved in regulating trophoblastic invasion of the uterus as well as in regulating remodeling of the extracellular matrix during the folding of epithelia, and in the formation, branching and expansion of epithelial tubes. TIMP-4 is most highly expressed in heart tissues. Studies have demonstrated that TIMP1 is useful as a biomarker for early detection of colorectal cancer, outperforming CEA. Additionally, TIMP1 studies have demonstrated its role in CRC tumorigenesis, as well as observing its overexpression in metastatic lymph nodes.

• Specifications:

  ELISA (order BSA-free format for coating)

• UniProt:

  P01033

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Amino acids 72-82 (FQALGDAADIR) were used as the immunogen for this TIMP1 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  2A5

• Applications:

  ELISA

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Store the TIMP1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This TIMP1 antibody is available for research use only.

• Storage Conditions:

  Store the TIMP1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the TIMP1 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Image Legend:

  SDS-PAGE analysis of purified, BSA-free TIMP1 antibody (clone 2A5) as confirmation of integrity and purity.