TIMP-2 Antibody

• Description :

  It recognizes a protein of 21kDa, identified as tissue inhibitor of metalloproteinases-2 (TIMP-2) . It is closely related to TIMP-1 and shows the highest binding affinity to both the latent (pro) and active forms of 72kDa Type IV collagenase (also known as MMP-2 or gelatinase A) . It also has affinity for the active form of 92kDa Type IV collagenase (also known as MMP-9 or gelatinase B) . TIMP-2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.

• Specifications :

  Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 0.5-1 µg/mL, Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

• UniProt :

  P16035

• Host :

  Mouse

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  Amino acids 56-66 (DSGNDIYGNPI) from the human protein were used as the immunogen for this TIMP-2 antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG2a κ

• Clone :

  3A4

• Applications :

  FACS, IF, WB, IHC-P

• Purity :

  Protein G affinity chromatography

• Format :

  Purified

• Buffer :

  1X PBS, pH 7.4

• Reconstitution :

  Store the TIMP-2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations :

  This TIMP-2 antibody is available for research use only.

• Storage Conditions :

  Store the TIMP-2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation :

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes :

  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TIMP-2 antibody to be titered up or down for optimal performance.

• CAS Number :

  9007-83-4

• Location :

  Cytoplasmic, cell surface, secreted

• Image Legend :

  IHC testing of FFPE human placenta with TIMP-2 antibody (clone 3A4) . Required HIER: requires boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.