TIMP-2 Antibody

• Description:

  It recognizes a protein of 21kDa, identified as tissue inhibitor of metalloproteinases-2 (TIMP-2) . It is closely related to TIMP-1 and shows the highest binding affinity to both the latent (pro) and active forms of 72kDa Type IV collagenase (also known as MMP-2 or gelatinase A) . It also has affinity for the active form of 92kDa Type IV collagenase (also known as MMP-9 or gelatinase B) . TIMP-2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.

• Specifications:

  Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 0.5-1 µg/mL, Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

• UniProt:

  P16035

• Host:

  Mouse

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Amino acids 56-66 (DSGNDIYGNPI) from the human protein were used as the immunogen for this TIMP-2 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2a κ

• Clone:

  3A4

• Applications:

  FACS, IF, WB, IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  1X PBS, pH 7.4

• Reconstitution:

  Store the TIMP-2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This TIMP-2 antibody is available for research use only.

• Storage Conditions:

  Store the TIMP-2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TIMP-2 antibody to be titered up or down for optimal performance.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic, cell surface, secreted

• Image Legend:

  IHC testing of FFPE human placenta with TIMP-2 antibody (clone 3A4) . Required HIER: requires boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.