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Bcl-2 Antibody

CAT: 0800-R32992Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R32992Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Immunoreactive BCL2 protein is in the neoplastic cells of almost all follicular lymphomas whereas no BCL2 protein was detected in follicles affected by nonneoplastic processes or in normal lymphoid tissue. Every tumor with molecular-genetic evidence of t (14;18) translocation expressed detectable levels of BCL2 protein, regardless of whether the breakpoint was located in or at a distance from the BCL2 gene. Overexpression of BCL2 blocks the apoptotic death of a pro-B-lymphocyte cell line.
CAS Number
9007-83-4
Specifications
Western Blot: 0.5-1 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL
UniProt
P10415
Host
Rabbit
Reactivity
Human
Immunogen
A recombinant human partial protein corresponding to amino acids Q118-E165 was used as the immunogen for the Bcl-2 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IF, FACS, ELISA
Purity
Antigen affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the Bcl-2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Bcl-2 antibody is available for research use only.
Storage Conditions
After reconstitution, the Bcl-2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the Bcl-2 antibody should be determined by the researcher.
Image Legend
Immunofluorescent staining of FFPE human U-2 OS cells with Bcl-2 antibody (red) and Alpha Tubulin antibody (green) . HIER: steam section in pH6 citrate buffer for 20 min.

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