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Bcl-X Antibody

CAT: 0800-V3121IHC-7MLSize: 7 mLDry Ice: NoHazardous: No
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CAT#:0800-V3121IHC-7MLSize:7 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recognizes a protein of 27kDa, identified as the Bcl-X protein. This mAb shows no cross-reaction with Bcl-2 or Bax protein. Bcl-X has two isoforms, Bcl-XL (long), a 241 amino acid protein which suppresses cell death. And Bcl-XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl-2. This mAb reacts with both Bcl-XS and Bcl-XL proteins.
CAS Number
9007-83-4
Specifications
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
UniProt
Q07817
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
Amino acids 3-14 (QSNRELVVDFLS) from the human protein were used as the immunogen for this Bcl-X antibody.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
2H12
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*
Reconstitution
Store the Bcl-X antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Bcl-X antibody is available for research use only.
Storage Conditions
Store the Bcl-X antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*
Location
Cytoplasmic and cell/nuclear membrane
Image Legend
Note cytoplasmic and membrane staining.

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