ACTA1 Antibody

• Description :

  Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures in the cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three alpha-actin, one beta-actin, two gamma-actin. Because the amino acid sequence of the C-terminal is the same for almost all actins, this antibody has been raised using a synthetic peptide corresponding to the C-terminal 11 residues.

• Specifications :

  Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL

• UniProt :

  P68133

• Host :

  Mouse

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  Amino acids HETTYNSIMKCDIDIRKDLYANNVMSGGTTMY from the human protein were used as the immunogen for the ACTA1 antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG1

• Clone :

  3H5

• Applications :

  WB, IHC-P

• Purity :

  Affinity purified

• Format :

  Antigen affinity purified

• Buffer :

  Lyophilized from 1X PBS with 2% Trehalose

• Reconstitution :

  After reconstitution, the ACTA1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations :

  This ACTA1 antibody is available for research use only.

• Storage Conditions :

  After reconstitution, the ACTA1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation :

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes :

  Optimal dilution of the ACTA1 antibody should be determined by the researcher.

• CAS Number :

  9007-83-4

• Image Legend :

  IHC staining of FFPE human bladder cancer with ACTA1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.