CD8 Antibody (alpha)

CAT: 0800-R31662Size: 100 µgDry Ice: NoHazardous: No
CAT#:0800-R31662Size:100 µg
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PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
CD8 is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. It is mapped to 2p11.2. CD8, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL
Host
Rabbit
Reactivity
Human
Immunogen
Human partial recombinant protein (AA 22-187) was used as the immunogen for this CD8 antibody. This amino acid sequence is from the alpha subunit.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Reconstitution
After reconstitution, the CD8 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This CD8 antibody is available for research use only.
Storage Conditions
After reconstitution, the CD8 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the CD8 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of CD8 antibody and Lane 1: Jurkat; 2: Raji; 3: HL-60; 4: A549; 5: COLO320; 6: HeLa lysate; Predicted molecular weight ~34 kDa; Observed molecular weight: 34~60 kDa depending on glycosylation level.
Product Datasheet
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